Academic Journal
Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins
| العنوان: | Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins |
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| المؤلفون: | Luiza Andreazza, Nathalia, Vevert-Bizet, Christine, Bourg Heckly, Genevieve, Sureau, Franck, Salvador, Marcos José, Bonneau, Stéphanie |
| المساهمون: | Instituto de Biologia, Departamento de Biologia Vegetal, Universidade Estadual de Campinas = University of Campinas (UNICAMP), Laboratoire Jean Perrin (LJP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0378-5173 ; International Journal of Pharmaceutics ; https://hal.science/hal-01399576 ; International Journal of Pharmaceutics, 2016, 510, pp.240 - 249. ⟨10.1016/j.ijpharm.2016.06.009⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Berberine, low-density lipoprotein, antitumor PDT, cellular uptake, subcellular localization, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] |
| الوصف: | International audience ; Recent years have seen a growing interest in Berberine, a phytochemical with multispectrum therapeutic activities, as anti-tumoral agent for photodynamic therapy (PDT). In this context, low density lipoproteins (LDL) play a key role in the delivery of the photosensitizer in tumor cells. We correlate the physicochemical parameters of the berberine association to LDL with the influence of LDL-delivery on its accumulation in a glioma cell line and on its photo-induced activity in view of antitumor PDT. Our results evidence an important binding of 400 berberine molecules per LDL. Changes in berberine and apoprotein fluorescence suggest different fixation types, involving various LDL compartments including the vicinity of the apoprotein. The berberine association to LDL does not affect their recognition by the specific B/E receptors, of which over-expression increases the cellular uptake of LDL-preloaded berberine. Fluorescence microscopy evidences the mitochondrial labeling of the glioma model cells, with no significant modification upon LDL-delivery. Moreover, the cellular delivery of berberine by LDL increases its photocytotoxic effects on such cells. So, this research illustrates the potential of berberine as a photosensitizing agent for PDT, in particular due to their behavior towards LDL as plasma vehicles, and gives insights into its mechanisms of cell uptake. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-01399576; https://hal.science/hal-01399576; https://hal.science/hal-01399576/document; https://hal.science/hal-01399576/file/Text-Abstract-GA-Legends_CORR-RELECTURE.pdf |
| DOI: | 10.1016/j.ijpharm.2016.06.009 |
| الاتاحة: | https://hal.science/hal-01399576 https://hal.science/hal-01399576/document https://hal.science/hal-01399576/file/Text-Abstract-GA-Legends_CORR-RELECTURE.pdf https://doi.org/10.1016/j.ijpharm.2016.06.009 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C94A9A6F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ijpharm.2016.06.009 |
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