Academic Journal
Gene expression profiling unravels cancer-related hepatic molecular signatures in steatohepatitis but not in steatosis.
| العنوان: | Gene expression profiling unravels cancer-related hepatic molecular signatures in steatohepatitis but not in steatosis. |
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| المؤلفون: | Julia Starmann, Maria Fälth, Walter Spindelböck, Katja-Lauren Lanz, Carolin Lackner, Kurt Zatloukal, Michael Trauner, Holger Sültmann |
| المصدر: | PLoS ONE, Vol 7, Iss 10, p e46584 (2012) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2012 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | BACKGROUND: Pathogenesis and factors for determining progression of alcoholic and non-alcoholic steatosis to steatohepatitis with risk of further progression to liver cirrhosis and cancer are poorly understood. In the present study, we aimed to identify potential molecular signatures for discrimination of steatohepatitis from steatosis. METHODOLOGY AND RESULTS: Global microarray gene expression analysis was applied to unravel differentially expressed genes between steatohepatitis compared to steatosis and control samples. For functional annotation as well as the identification of disease-relevant biological processes of the differentially expressed genes the gene ontology (GO) database was used. Selected candidate genes (n = 46) were validated in 87 human liver samples from two sample cohorts by quantitative real-time PCR (qRT-PCR). The GO analysis revealed that genes down-regulated in steatohepatitis were mainly involved in metabolic processes. Genes up-regulated in steatohepatitis samples were associated with cancer progression and proliferation. In surgical liver resection samples, 39 genes and in percutaneous liver biopsies, 30 genes were significantly up-regulated in steatohepatitis. Furthermore, immunohistochemical investigation of human liver tissue revealed a significant increase of AKR1B10 protein expression in steatohepatitis. CONCLUSIONS: The development of steatohepatitis is characterized by distinct molecular changes. The most striking examples in this respect were KRT23 and AKR1B10, which we found to be highly differentially expressed in steatohepatitis compared to steatosis and normal liver. We propose that KRT23 and AKR1B10 may serve as future potential biomarkers for steatohepatitis as well as markers for progression to HCC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3468618?pdf=render; https://doaj.org/toc/1932-6203; https://doaj.org/article/83884fafb97d409aaa686f1eec04a32b |
| DOI: | 10.1371/journal.pone.0046584 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0046584 https://doaj.org/article/83884fafb97d409aaa686f1eec04a32b |
| رقم الانضمام: | edsbas.C8CAC9E1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0046584 |