Academic Journal
Neuromelanins of human brain have soluble and insoluble components with dolichols attached to the melanic structure.
| العنوان: | Neuromelanins of human brain have soluble and insoluble components with dolichols attached to the melanic structure. |
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| المؤلفون: | Mireille Engelen, Renzo Vanna, Chiara Bellei, Fabio A Zucca, Kazumasa Wakamatsu, Enrico Monzani, Shosuke Ito, Luigi Casella, Luigi Zecca |
| المصدر: | PLoS ONE, Vol 7, Iss 11, p e48490 (2012) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2012 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Neuromelanins (NMs) are neuronal pigments of melanic-lipidic type which accumulate during aging. They are involved in protective and degenerative mechanisms depending on the cellular context, however their structures are still poorly understood. NMs from nine human brain areas were analyzed in detail. Elemental analysis led to identification of three types of NM, while infrared spectroscopy showed that NMs from neurons of substantia nigra and locus coeruleus, which selectively degenerate in Parkinson's disease, have similar structure but different from NMs from brain regions not targeted by the disease. Synthetic melanins containing Fe and bovine serum albumin were prepared to model the natural product and help clarifying the structure of NMs. Extensive nuclear magnetic resonance spectroscopy studies showed the presence of dolichols both in the soluble and insoluble parts of NM. Diffusion measurements demonstrated that the dimethyl sulfoxide soluble components consist of oligomeric precursors with MWs in the range 1.4-52 kDa, while the insoluble part contains polymers of larger size but with a similar composition. These data suggest that the selective vulnerability of neurons of substantia nigra and locus coeruleus in Parkinson's disease might depend on the structure of the pigment. Moreover, they allow to propose a pathway for NM biosynthesis in human brain. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139786/pdf/?tool=EBI; https://doaj.org/toc/1932-6203; https://doaj.org/article/612df313c4f54d11a2c1062e9c1b1463 |
| DOI: | 10.1371/journal.pone.0048490 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0048490 https://doaj.org/article/612df313c4f54d11a2c1062e9c1b1463 |
| رقم الانضمام: | edsbas.C8C7D51B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0048490 |