التفاصيل البيبلوغرافية
| العنوان: |
Antioxidant activity of ethanolic extract of inflorescence of Ormenis Africanain vitro and in cell cultures |
| المؤلفون: |
Ben Mansour, Riadh, Gargouri, Bochra, Bouaziz, Mohamed, Elloumi, Nésrine, Belhadj Jilani, Imtinène, Ghrabi, Zaineb, Lassoued, Saloua |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2011 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background The antioxidant potency of the hydroethanolic extract of Ormenis Africana (HEOA), Asteraceae was evaluated with regards to total polyphenol, flavonoid and anthocyanins content. Antioxidant activity has been assessed chemically and biologically. First, the free radical scavenging ability of HEOA was evaluated using two commonly in vitro tests: ABTS and DPPH radicals. Then, the protection effect of this extract against oxidative stress was conducted in HeLa cells treated with Fe 2+ or H 2 O 2. Oxidative stress was evaluated by measuring the lipid peroxidation levels (TBARs and DC) and the antioxidant enzymes activities (catalase and Superoxide dismutase). Cytotoxic effect of HEOA was prealably determined against HeLa cell line by MTT assay. Results HEOA contain considerable levels of antioxidant compound as evidenced by high amount of polyphenols (312.07 mg GAE/g dray matter), flavonoids (73.72 ± 1.98 mg QE/g dray matterl) and anthocyanins (0.28 ± 0.09 mg Cy-3-glu E/g dray matter). DPPH and ABTS assays showed a high antioxidant activity (IC 50 = 24 μg/ml; TEAC = 2.137 mM) which was comparable to BHT. In biological system, HEOA exhibited a 50% cytotoxic concentration evaluated as 16.52 μg/ml. Incubation of HeLa cell line with no cytotoxic concentrations resulted in a remarkable protection from oxidative stress induced by Fe 2+ or H 2 O 2 which was evidenced by a decrease of MDA and CD levels as well as a diminution of antioxidant enzymes activities (Catalase and SOD) as compared to cells treated with Fe 2+ or H 2 O 2 alone. Conclusion The hydroethanolic extract of O. Africana could thus be considered as a source of potential antioxidants. The results of this study will promote the reasonable usage of this plant in food and pharmacy industries as well as in alternative medicine and natural therapy. |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| Relation: |
http://www.lipidworld.com/content/10/1/78 |
| الاتاحة: |
http://www.lipidworld.com/content/10/1/78 |
| Rights: |
Copyright 2011 Ben Mansour et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.C8ACAC64 |
| قاعدة البيانات: |
BASE |