Academic Journal
Molecular Mapping of Hydrogen Sulfide Targets in Normal Human Keratinocytes
| العنوان: | Molecular Mapping of Hydrogen Sulfide Targets in Normal Human Keratinocytes |
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| المؤلفون: | Gross-Amat, O., Guillen, M., Gimeno, J. P., Salzet, M., Lebonvallet, N., Misery, L., Auxenfans, C., Nataf, S. |
| المساهمون: | Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Hôpital de la Cavale Blanche - CHRU Brest (CHU - BREST ), Banque de tissus et de cellules, Hôpital Edouard Herriot CHU - HCL, Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French association for research on spa waters (AFRETH: "Association Francaise pour la Recherche Thermale") |
| المصدر: | Int. J. Mol. Sci. ; https://hal.science/hal-02902509 ; Int. J. Mol. Sci., 2020, 21 (13), pp.4648. ⟨10.3390/ijms21134648⟩ |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2020 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | H2s, Human keratinocyte, Proteomics, RNAseq, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Although sulfur-rich thermal waters have ancestrally been used in the context of dermatological conditions, a global mapping of the molecular effects exerted by H(2)S on human keratinocytes is still lacking. To fill this knowledge gap, we subjected cultured human keratinocytes to distinct amounts of the non-gaseous hydrogen sulfur donor NaHS. We first checked that H(2)S accumulated in the cytoplasm of keratinocytes under our experimental conditions andused a combination of proteomics, genomics and biochemical approaches to unravel functionally relevant H(2)S targets in human keratinocytes. We found that the identified targets fall into two main categories: (i) the oxidative stress response molecules superoxide dismutase 2 (SOD2), NAD(P)H quinone dehydrogenase 1 (NQO1) and culin 3 (CUL3) and (ii) the chemokines interleukin-8 (IL-8) and CXCL2. Interestingly, NaHS also stimulated the caspase-1 inflammasome pathway, leading to increased secretion of the pro-inflammatory molecule interleukin-18 (IL-18). Interestingly, the secretion of interleukin-1 beta (IL-1β) was only modestly impacted by NaHS exposure despite a significant accumulation of IL-1β pro-form. Finally, we observed that NaHS significantly hampered the growth of human keratinocyte progenitors and stem cells cultured under clonogenic conditions or as epidermal cell sheets. We conclude that H(2)S exerts specific molecular effects on normal human keratinocytes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32629886; hal-02902509; https://hal.science/hal-02902509; https://hal.science/hal-02902509/document; https://hal.science/hal-02902509/file/ijms-21-04648-v2.pdf; PUBMED: 32629886; PUBMEDCENTRAL: PMC7369889; WOS: 000550233700001 |
| DOI: | 10.3390/ijms21134648 |
| الاتاحة: | https://hal.science/hal-02902509 https://hal.science/hal-02902509/document https://hal.science/hal-02902509/file/ijms-21-04648-v2.pdf https://doi.org/10.3390/ijms21134648 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C88ABEC6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms21134648 |
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