Dissertation/ Thesis
Expression and roles of pericentric heterochromatin in cellular response to heat stress ; Expression et rôles de l'héterochromatine péricentrique humaine au cours de la réponse cellulaire au stress thermique
| العنوان: | Expression and roles of pericentric heterochromatin in cellular response to heat stress ; Expression et rôles de l'héterochromatine péricentrique humaine au cours de la réponse cellulaire au stress thermique |
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| المؤلفون: | Dufour, Solenne |
| المساهمون: | Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Grenoble Alpes 2020-., Claire Vourc'h |
| المصدر: | https://theses.hal.science/tel-04368494 ; Organisation et fonctions cellulaires [q-bio.SC]. Université Grenoble Alpes [2020-.], 2022. Français. ⟨NNT : 2022GRALV047⟩. |
| بيانات النشر: | CCSD |
| سنة النشر: | 2022 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | Non coding RNA, Stress, Heterochromatin, Hsf1, SATII RNA, Nuclear stress bodies, ARNs non codants, Hétérochromatine, ARN SATIII, Foyers de stress nucléaires, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | The signaling pathway controlled by the transcription factor HSF1 (Heat Shock Factor 1) has been extensively studied in the literature.Its activation is essential for the survival of cells under stress. The HSF1 factor has so far been particularly studied for its impact on the coding fraction of the genome, but we now know that it is also capable of inducing the synthesis of non-coding RNAs, satellite type III (SATIII RNA) in humans. The function of these RNAs, which are very abundantly transcribed in stressed cells, is still poorly understood. They are produced from pericentric heterochromatin regions of several megabases, which are in principle transcriptionally silent in unstressed cells. My PhD dissertation is focused on the characterisation of the structure and function of SATIII RNAs derived from these regions.Our objective was first to better understand the nature of these transcripts as well as the molecular mechanisms associated with their transcription and function. Using in situ approaches, we also sought to better characterise the epigenetic status of pericentric regions during the stress response. We have identified a particular expression profile related to the epigenetic status of these regions during their transient activation, as well as the existence of a major new target located on the Y chromosome. Our results show that the accumulation of these RNAs correlates with the transient loss of repressive marks, the arrest of the G2/M/G1 transition in stressed cells, and induce mitotic defects in stressed HeLa cells. Finally, the use of strategies to decrease the level of these RNAs highlights a new and essential role for these RNAs in both stressed and unstressed cells. ; La voie de signalisation contrôlée par le facteur de transcription HSF1 (pour Heat Shock Factor 1) a été beaucoup étudiée dans la littérature.Son activation est essentielle à la survie des cellules soumises à un stress. Le facteur HSF1 a été jusqu’à présent particulièrement étudié pour son impact sur la fraction codante du génome ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | French |
| Relation: | NNT: 2022GRALV047 |
| الاتاحة: | https://theses.hal.science/tel-04368494 https://theses.hal.science/tel-04368494v1/document https://theses.hal.science/tel-04368494v1/file/DUFOUR_2022_archivage.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C8647363 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |