Academic Journal
Specific IgE and Basophil Activation Test by Microarray: A Promising Tool for Diagnosis of Platinum Compound Hypersensitivity Reactions
| العنوان: | Specific IgE and Basophil Activation Test by Microarray: A Promising Tool for Diagnosis of Platinum Compound Hypersensitivity Reactions |
|---|---|
| المؤلفون: | Carlos Fernández-Lozano, Claudia Geraldine Rita, Alicia Barra-Castro, Belén de la Hoz Caballer, Ernesto Roldán, Cristina Pueyo López, Javier Martinez-Botas, María Pilar Berges-Gimeno |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 7, p 3890 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | basophil activation test, drug allergies, hypersensitivity reaction, microarray, platinum compounds, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Drug hypersensitivity reactions (DHRs) to platinum-based compounds (PCs) are on the rise, and their personalized and safe management is essential to enable first-line treatment for these cancer patients. This study aimed to evaluate the usefulness of the basophil activation test by flow cytometry (BAT-FC) and the newly developed sIgE-microarray and BAT-microarray in diagnosing IgE-mediated hypersensitivity reactions to PCs. A total of 24 patients with DHRs to PCs (20 oxaliplatin and four carboplatin) were evaluated: thirteen patients were diagnosed as allergic with positive skin tests (STs) or drug provocation tests (DPTs), six patients were diagnosed as non-allergic with negative STs and DPTs, and five patients were classified as suspected allergic because DPTs could not be performed. In addition, four carboplatin-tolerant patients were included as controls. The BAT-FC was positive in 2 of 13 allergic patients, with a sensitivity of 15.4% and specificity of 100%. However, the sIgE- and BAT-microarray were positive in 11 of 13 DHR patients, giving a sensitivity of over 84.6% and a specificity of 90%. Except for one patient, all samples from the non-allergic and control groups were negative for sIgE- and BAT-microarray. Our experience indicated that the sIgE- and BAT-microarray could be helpful in the endophenotyping of IgE-mediated hypersensitivity reactions to PCs and may provide an advance in decision making for drug provocation testing. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/7/3890; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/1006b0d229ff44b69de4727f23dedaf0 |
| DOI: | 10.3390/ijms25073890 |
| الاتاحة: | https://doi.org/10.3390/ijms25073890 https://doaj.org/article/1006b0d229ff44b69de4727f23dedaf0 |
| رقم الانضمام: | edsbas.C84F9F6F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
|---|---|
| DOI: | 10.3390/ijms25073890 |