Academic Journal
Critical role for Piccolo in synaptic vesicle retrieval.
| العنوان: | Critical role for Piccolo in synaptic vesicle retrieval. |
|---|---|
| المؤلفون: | Ackermann, Frauke, Schink, Kay Oliver, Bruns, Christine, Izsvák, Zsuzsanna, Hamra, F Kent, Rosenmund, Christian, Garner, Craig Curtis |
| المصدر: | eLife 8, e46629 (2019). doi:10.7554/eLife.46629 |
| بيانات النشر: | eLife Sciences Publications |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/600, Animals, Axons: metabolism, Cytoskeletal Proteins: metabolism, DNA Transposable Elements: genetics, Endosomes: metabolism, GTP Phosphohydrolases: metabolism, Gene Knockout Techniques, Guanosine Diphosphate: metabolism, Models, Biological, Mutagenesis: genetics, Neuropeptides: metabolism, Phosphatidylinositol Phosphates: metabolism, Rats, Synaptic Vesicles: metabolism, Synaptic Vesicles: ultrastructure, Synaptophysin: metabolism, Vesicular Transport Proteins: metabolism, rab5 GTP-Binding Proteins: metabolism |
| جغرافية الموضوع: | DE |
| الوصف: | Loss of function of the active zone protein Piccolo has recently been linked to a disease, Pontocerebellar Hypoplasia type 3, which causes brain atrophy. Here, we address how Piccolo inactivation in rat neurons adversely affects synaptic function and thus may contribute to neuronal loss. Our analysis shows that Piccolo is critical for the recycling and maintenance of synaptic vesicles. We find that boutons lacking Piccolo have deficits in the Rab5/EEA1 dependent formation of early endosomes and thus the recycling of SVs. Mechanistically, impaired Rab5 function was caused by reduced synaptic recruitment of Pra1, known to interact selectively with the zinc finger domains of Piccolo. Importantly, over-expression of GTPase deficient Rab5 or the Znf1 domain of Piccolo restores the size and recycling of SV pools. These data provide a molecular link between the active zone and endosome sorting at synapses providing hints to how Piccolo contributes to developmental and psychiatric disorders. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/pmid:31074746; info:eu-repo/semantics/altIdentifier/issn/2050-084X; https://pub.dzne.de/record/140720; https://pub.dzne.de/search?p=id:%22DZNE-2020-07042%22 |
| الاتاحة: | https://pub.dzne.de/record/140720 https://pub.dzne.de/search?p=id:%22DZNE-2020-07042%22 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.C839DBB5 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |