التفاصيل البيبلوغرافية
| العنوان: |
Feasibility of Direct Vitrectomy-Sparing Subretinal Injection for Gene Delivery in Large Animals |
| المؤلفون: |
Stranak, Zbynek, Ardan, Taras, Nemesh, Yaroslav, Toms, Maria, Toualbi, Lyes, Harbottle, Richard, Ellederova, Zdenka, Lytvynchuk, Lyubomyr, Petrovski, Goran, Motlik, Jan, Moosajee, Mariya, Kozak, Igor |
| المصدر: |
Current Eye Research , 49 (8) pp. 879-887. (2024) |
| بيانات النشر: |
TAYLOR & FRANCIS INC |
| سنة النشر: |
2024 |
| المجموعة: |
University College London: UCL Discovery |
| مصطلحات موضوعية: |
Subretinal injection, pars planavitrectomy, large animal, non-viralgene vector, safety |
| الوصف: |
Purpose: To assess the safety and feasibility of direct vitrectomy-sparing subretinal injection for gene delivery in a large animal model. // Methods: The experimental Liběchov minipigs were used for subretinal delivery of a plasmid DNA vector (pS/MAR-CMV-copGFP) with cytomegalovirus (CMV) promoter, green fluorescent protein (GFP) reporter (copGFP) and a scaffold/matrix attachment region (S/MAR) sequence. The eyes were randomized to subretinal injection of the vector following pars plana vitrectomy (control group) or a direct injection without prior vitrectomy surgery (experimental group). Intra- and post-operative observations up to 30 days after surgery were compared. // Results: Six eyes of three mini-pigs underwent surgery for delivery into the subretinal space. Two eyes in the control group were operated with a classical approach (lens-sparing vitrectomy and posterior hyaloid detachment). The other four eyes in the experimental group were injected directly with a subretinal cannula without vitrectomy surgery. No adverse events, such as endophthalmitis, retinal detachment and intraocular pressure elevation were observed post-operatively. The eyes in the experimental group had both shorter surgical time and recovery while achieving the same surgical goal. // Conclusions: This pilot study demonstrates that successful subretinal delivery of gene therapy vectors is achievable using a direct injection without prior vitrectomy surgery. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
https://discovery.ucl.ac.uk/id/eprint/10203088/ |
| الاتاحة: |
https://discovery.ucl.ac.uk/id/eprint/10203088/ |
| رقم الانضمام: |
edsbas.C7A2BB74 |
| قاعدة البيانات: |
BASE |