Human mesenchymal stem cells enhance the systemic effects of radiotherapy.

التفاصيل البيبلوغرافية
العنوان:	Human mesenchymal stem cells enhance the systemic effects of radiotherapy.
المؤلفون:	de Araújo Farias, Virgínea, O'Valle, Francisco, Lerma, Borja Alonso, Ruiz de Almodóvar, Carmen, López-Peñalver, Jesús J, Nieto, Ana, Santos, Ana, Fernández, Beatriz Irene, Guerra-Librero, Ana, Ruiz-Ruiz, María Carmen, Guirado, Damián, Schmidt, Thomas, Oliver, Francisco Javier, Ruiz de Almodóvar, José Mariano
المساهمون:	de Araújo Farias,V, O'Valle,F, Lerma,BA, López-Peñalver,JJ, Nieto,A, Santos,A, Fernández,BI, Guerra-Librero,A, Ruiz-Ruiz,MC, Ruiz de Almodóvar,JM Instituto Universitario de Investigación en Biopatología y Medicina Regenerativa, Centro de Investigación Biomédica, Universidad de Granada, Granada, Spain. de Araújo Farias,V, Oliver,FJ Instituto de Parasitología y Biomedicina “López Neyra”, Consejo Superior de Investigaciones Científicas, Granada, Spain. Ruiz de Almodóvar,C Biochemistry Center, University of Heidelberg, Heidelberg, Germany. López-Peñalver,JJ Unidad de radiología experimental, Centro de Instrumentación Científica, Centro de Investigación Biomédica, Universidad de Granada, Granada, Spain. Nieto,A Unidad de Experimentación Animal, Centro de Instrumentación Científica, Centro de Investigación Biomédica, Universidad de Granada, Granada, Spain. Santos,A Unidad de Microscopia, Centro de Instrumentación Científica, Centro de Investigación Biomédica, Universidad de Granada, Granada, Spain. Ruiz-Ruiz,MC Departamento de Bioquímica y Biología Molecular III e Inmunología, IBIMER, Centro de Investigación Biomédica, Universidad de Granada, Granada, Spain. Guirado,D, Ruiz de Almodóvar,JM Hospital Universitario San Cecilio, Granada, Spain. Schmidt,T Department of General, Visceral and Transplant Surgery, University of Heidelberg, Heidelberg, Germany., This work was supported by Ministerio de Economía y Competividad, MINECO: SAF2012-40011-C02-02 to JMRdA, and MINECO SAF2012-40011-CO2-01 to F.J.O and CNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico - Brasil.
بيانات النشر:	Impact Journals
سنة النشر:	2015
المجموعة:	Sistema Sanitario Público de Andalucía (SSPA): Repositorio
مصطلحات موضوعية:	Radiotherapy, Bystander effect, Mesenchymal cells, Cancer, Radiosensitizers, Western Blotting, Proliferación de la célula, Radioisótopos de cesio, Humanos, Técnicas para inmunoenzimas, Trasplante de células madre mesenquimatosas, Células del estroma mesenquimatoso, Ratones, Ratones SCID, Reacción en cadena en tiempo real de la polimerasa, Reacción en cadena de la polimerasa de transcriptasa inversa, Células tumorales cultivadas, Ensayos antitumor por modelo de xenoinjerto, Animales, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis, Medical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Blotting, Western, Medical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation, Medical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Isotopes::Cesium Isotopes::Cesium Radioisotopes, Medical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoenzyme Techniques, Diagnostic and Therapeutic Techniques and Equipment::Surgical Procedures, Operative::Transplantation::Cell Transplantation::Stem Cell Transplantation::Mesenchymal Stem Cell Transplantation
الوصف:	Journal Article; Research Support, Non-U.S. Gov't; ; The outcome of radiotherapy treatment might be further improved by a better understanding of individual variations in tumor radiosensitivity and normal tissue reactions, including the bystander effect. For many tumors, however, a definitive cure cannot be achieved, despite the availablity of more and more effective cancer treatments. Therefore, any improvement in the efficacy of radiotherapy will undoubtedly benefit a significant number of patients. Many experimental studies measure a bystander component of tumor cell death after radiotherapy, which highlights the importance of confirming these observations in a preclinical situation. Mesenchymal stem cells (MSCs) have been investigated for use in the treatment of cancers as they are able to both preferentially home onto tumors and become incorporated into their stroma. This process increases after radiation therapy. In our study we show that in vitro MSCs, when activated with a low dose of radiation, are a source of anti-tumor cytokines that decrease the proliferative activity of tumor cells, producing a potent cytotoxic synergistic effect on tumor cells. In vivo administration of unirradiated mesenchymal cells together with radiation leads to an increased efficacy of radiotherapy, thus leading to an enhancement of short and long range bystander effects on primary-irradiated tumors and distant-non-irradiated tumors. Our experiments indicate an increased cell loss rate and the decrease in the tumor cell proliferation activity as the major mechanisms underlying the delayed tumor growth and are a strong indicator of the synergistic effect between RT and MSC when they are applied together for tumor treatment in this model. ; Yes
نوع الوثيقة:	article in journal/newspaper
وصف الملف:	application/pdf
اللغة:	English
تدمد:	1949-2553
Relation:	http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=5216&pubmed-linkout=1; de Araújo Farias V, O'Valle F, Lerma BA, Ruiz de Almodóvar C, López-Peñalver JJ, Nieto A, et al. Human mesenchymal stem cells enhance the systemic effects of radiotherapy. Oncotarget. 2015; 6(31):31164-80; http://hdl.handle.net/10668/2566; PMC4741595
DOI:	10.18632/oncotarget.5216
الاتاحة:	http://hdl.handle.net/10668/2566 https://doi.org/10.18632/oncotarget.5216
Rights:	open access
رقم الانضمام:	edsbas.C784B814
قاعدة البيانات:	BASE

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الوصف
تدمد:	19492553
DOI:	10.18632/oncotarget.5216