Academic Journal
Arabinosylation of cell wall extensin is required for the directional response to salinity in roots
| العنوان: | Arabinosylation of cell wall extensin is required for the directional response to salinity in roots |
|---|---|
| المؤلفون: | Zou, Y., Gigli-Bisceglia, N., van Zelm, E., Kokkinopoulou, P., Julkowska, M.M., Besten, M., Nguyen, T.-P., Li, H., Lamers, J., de Zeeuw, T., Dongus, J.A., Zeng, Y., Cheng, Y., Koevoets, I.T., Jørgensen, B., Giesbers, M., Vroom, J., Ketelaar, T., Petersen, B.L., Engelsdorf, T., Sprakel, J., Zhang, Y., Testerink, C. |
| المصدر: | Zou , Y , Gigli-Bisceglia , N , van Zelm , E , Kokkinopoulou , P , Julkowska , M M , Besten , M , Nguyen , T-P , Li , H , Lamers , J , de Zeeuw , T , Dongus , J A , Zeng , Y , Cheng , Y , Koevoets , I T , Jørgensen , B , Giesbers , M , Vroom , J , Ketelaar , T , Petersen , B L , Engelsdorf , T , Sprakel , J , Zhang , Y & Testerink , C 2024 , .... |
| سنة النشر: | 2024 |
| المجموعة: | Universiteit van Amsterdam: Digital Academic Repository (UvA DARE) |
| الوصف: | Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-Time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na + ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O -glycosylation, mostly involving Hyp-Arabinosylation. We show that salt-induced ExAD-dependent Hyp-Arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-Arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-Avoidance response. Our results suggest that extensin modification via Hyp-Arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1093/plcell/koae135 |
| الاتاحة: | https://dare.uva.nl/personal/pure/en/publications/arabinosylation-of-cell-wall-extensin-is-required-for-the-directional-response-to-salinity-in-roots(e982467a-6b87-4f88-8ac3-53d0fb37aeb2).html https://doi.org/10.1093/plcell/koae135 https://hdl.handle.net/11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2 https://pure.uva.nl/ws/files/215275221/Arabinosylation_of_cell_wall_extensin_is_required.pdf http://www.scopus.com/inward/record.url?scp=85203247943&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C761264C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/plcell/koae135 |
|---|