Academic Journal
Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells
| العنوان: | Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells |
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| المؤلفون: | Bodak, Maxime, Cirera-Salinas, Daniel, Yu, Jian, Ngondo, Richard P., Ciaudo, Constance |
| المصدر: | FEBS Open Bio, 7 (2) |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | ETH Zürich Research Collection |
| مصطلحات موضوعية: | Dicer, LINE-1 retrotransposition, mouse embryonic stem cells, transposable elements |
| الوصف: | gene regulation network orchestrates processes ensuring the maintenance of cellular identity and genome integrity. Small RNAs generated by the RNAse III DICER have emerged as central players in this network. Moreover, deletion of Dicer in mice leads to early embryonic lethality. To better understand the underlying mechanisms leading to this phenotype, we generated Dicer-deficient mouse embryonic stem cells (mESCs). Their detailed characterization revealed an impaired differentiation potential, and incapacity to exit from the pluripotency state. We also observed a strong accumulation of LINE-1 (L1s) transcripts, which was translated at protein level and led to an increased L1s retrotransposition. Our findings reveal Dicer as a new essential player that sustains mESCs self-renewal and genome integrity by controlling L1s regulation. ; ISSN:2211-5463 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/000397220400008; info:eu-repo/grantAgreement/SNF/Projektförderung in Biologie und Medizin (Abteilung III)/153220; http://hdl.handle.net/20.500.11850/238068 |
| DOI: | 10.3929/ethz-b-000235911 |
| الاتاحة: | https://hdl.handle.net/20.500.11850/238068 https://doi.org/10.3929/ethz-b-000235911 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ ; Creative Commons Attribution 4.0 International |
| رقم الانضمام: | edsbas.C760BF0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000235911 |
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