التفاصيل البيبلوغرافية
| العنوان: |
Structural Basis of Transcriptional Gene Silencing Mediated by Arabidopsis MOM1 |
| المؤلفون: |
Taisuke Nishimura ¤a¤b, Guillaume Molinard, Tom J. Petty, Larissa Broger, Caroline Gabus, Thanos D, Stéphane Thore, Jerzy Paszkowski |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/a4/1c/PLoS_Genet_2012_Feb_9_8(2)_e1002484.tar.gz |
| سنة النشر: |
2011 |
| المجموعة: |
CiteSeerX |
| الوصف: |
Shifts between epigenetic states of transcriptional activity are typically correlated with changes in epigenetic marks. However, exceptions to this rule suggest the existence of additional, as yet uncharacterized, layers of epigenetic regulation. MOM1, a protein of 2,001 amino acids that acts as a transcriptional silencer, represents such an exception. Here we define the 82 amino acid domain called CMM2 (Conserved MOM1 Motif 2) as a minimal MOM1 fragment capable of transcriptional regulation. As determined by X-ray crystallography, this motif folds into an unusual hendecad-based coiled-coil. Structurebased mutagenesis followed by transgenic complementation tests in plants demonstrate that CMM2 and its dimerization are effective for transcriptional suppression at chromosomal loci co-regulated by MOM1 and the siRNA pathway but not at loci controlled by MOM1 in an siRNA–independent fashion. These results reveal a surprising separation of epigenetic |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.271.9960 |
| الاتاحة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.271.9960 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.C719D133 |
| قاعدة البيانات: |
BASE |