التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of intra- and extra-epithelial secretory IgA in chlamydial infections |
| المؤلفون: |
Armitage, Charles, O'Meara, Connor, Harvie, Marina, Timms, Peter, Wijburg, Odilia, Beagley, Kenneth |
| المصدر: |
Immunology |
| بيانات النشر: |
Wiley-Blackwell Publishing Ltd |
| سنة النشر: |
2014 |
| المجموعة: |
Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: |
Chlamydia, IgA, Immunology, polymeric Ig receptor, vaccine |
| الوصف: |
IgA is an important mucosal antibody that can neutralize mucosal pathogens by either preventing attachment to epithelia (immune exclusion) or alternatively inhibit intraepithelial replication following transcytosis by the polymeric immunoglobulin receptor (pIgR). Chlamydia trachomatis is a major human pathogen that initially targets the endocervical or urethral epithelium in women and men, respectively. As both tissues contain abundant SIgA we assessed the protection afforded by IgA targeting different chlamydial antigens expressed during the extra and intraepithelial stages of infection. We developed an in vitro model utilizing polarizing cells expressing the murine pIgR together with antigen-specific mouse IgA, and an in vivo model utilizing pIgR-/- mice. SIgA targeting the extraepithelial chlamydial antigen, the major outer membrane protein (MOMP), significantly reduced infection in vitro by 24 % and in vivo by 44 %. Conversely, pIgR-mediated delivery of IgA targeting the intraepithelial inclusion membrane protein A (IncA) bound to the inclusion but did not reduce infection in vitro or in vivo. Similarly, intraepithelial IgA targeting the secreted protease Chlamydia protease-like activity factor (CPAF) also failed to reduce infection. Together, these data suggest the importance of pIgR-mediated delivery of IgA targeting extra but not intraepithelial chlamydial antigens for protection against a genital tract infection. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| Relation: |
https://eprints.qut.edu.au/82614/3/pIgR%2520Manuscript_amended%2520x.pdf; Armitage, Charles, O'Meara, Connor, Harvie, Marina, Timms, Peter, Wijburg, Odilia, & Beagley, Kenneth (2014) Evaluation of intra- and extra-epithelial secretory IgA in chlamydial infections. Immunology, 143(4), pp. 520-530.; https://eprints.qut.edu.au/82614/; Faculty of Health; Institute of Health and Biomedical Innovation |
| الاتاحة: |
https://eprints.qut.edu.au/82614/ |
| Rights: |
free_to_read ; Consult author(s) regarding copyright matters ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: |
edsbas.C707F2EB |
| قاعدة البيانات: |
BASE |