Academic Journal
Characterization of plant microRNA-encoded peptides (miPEPs) reveals molecular mechanisms from the translation to activity and specificity
| العنوان: | Characterization of plant microRNA-encoded peptides (miPEPs) reveals molecular mechanisms from the translation to activity and specificity |
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| المؤلفون: | Lauressergues, Dominique, Ormancey, Mélanie, Guillotin, Bruno, San Clemente, Hélène, Camborde, Laurent, Duboé, Carine, Tourneur, Sabine, Charpentier, Pierre, Barozet, Amélie, Jauneau, Alain, Le Ru, Aurélie, Thuleau, Patrice, Gervais, Virginie, Plaza, Serge, Combier, Jean-Philippe |
| المساهمون: | Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Interactions Microbiennes dans la Rhizosphère et les Racines, Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Peptides et petits ARN, Micropep Technologies, Plateforme TRI FR-AIB, Fédération de Recherche Agrobiosciences, Interactions et Biodiversité (FR AIB), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de pharmacologie et de biologie structurale (IPBS), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE12-0018,biomiPEPs,Vers une compréhension globale des miPEPs(2016), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010) |
| المصدر: | ISSN: 2211-1247. |
| بيانات النشر: | HAL CCSD Elsevier Inc |
| سنة النشر: | 2022 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | miRNAs, miPEPs, peptides, Arabidopsis, Brassicacea, MESH: Arabidopsis / metabolism, MESH: Gene Expression Regulation, Plant / genetics, MESH: MicroRNAs / metabolism, MESH: Open Reading Frames / genetics, MESH: Peptides / metabolism, MESH: Plants / genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
| الوصف: | International audience ; MicroRNAs (miRNAs) are transcribed as long primary transcripts (pri-miRNAs) by RNA polymerase II. Plant pri-miRNAs encode regulatory peptides called miPEPs, which specifically enhance the transcription of the pri-miRNA from which they originate. However, paradoxically, whereas miPEPs have been identified in different plant species, they are poorly conserved, raising the question of the mechanisms underlying their specificity. To address this point, we identify and re-annotate multiple Arabidopsis thaliana pri-miRNAs in order to identify ORF encoding miPEPs. The study of several identified miPEPs in different species show that non-conserved miPEPs are only active in their plant of origin, whereas conserved ones are active in different species. Finally, we find that miPEP activity relies on the presence of its own miORF, explaining both the lack of selection pressure on miPEP sequence and the ability for non-conserved peptides to play a similar role, i.e., to activate the expression of their corresponding miRNA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35139385; hal-03602110; https://ut3-toulouseinp.hal.science/hal-03602110; https://ut3-toulouseinp.hal.science/hal-03602110/document; https://ut3-toulouseinp.hal.science/hal-03602110/file/Lauressergues_2022.pdf; PUBMED: 35139385 |
| DOI: | 10.1016/j.celrep.2022.110339 |
| الاتاحة: | https://ut3-toulouseinp.hal.science/hal-03602110 https://ut3-toulouseinp.hal.science/hal-03602110/document https://ut3-toulouseinp.hal.science/hal-03602110/file/Lauressergues_2022.pdf https://doi.org/10.1016/j.celrep.2022.110339 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C5F79089 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.celrep.2022.110339 |
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