Academic Journal
A New Bifidobacteria Expression SysTem (BEST) to Produce and Deliver Interleukin-10 in Bifidobacterium bifidum
| العنوان: | A New Bifidobacteria Expression SysTem (BEST) to Produce and Deliver Interleukin-10 in Bifidobacterium bifidum |
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| المؤلفون: | Mauras, Aurélie, Chain, Florian, Faucheux, Aurélie, Ruffié, Pauline, Gontier, Sophie, Ryffel, Bernhard, Butel, Marie-José, Langella, Philippe, Bermúdez-Humarán, Luis, Waligora-Dupriet, Anne-Judith |
| المساهمون: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Immunologie et Neurogénétique Expérimentales et Moléculaires (INEM), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), Faculté de Pharmacie de Paris - Université Paris Descartes (UPD5 Pharmacie) |
| المصدر: | ISSN: 1664-302X. |
| بيانات النشر: | CCSD Frontiers Media |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | recombinant bacteria, heterologous expression system, low-grade intestinal inflammation, bifidobacterium bifidum, microbiota, il-10, [SDV]Life Sciences [q-bio], [SDV.IMM]Life Sciences [q-bio]/Immunology |
| الوصف: | International audience ; In the last years there has been a growing interest in the use of genetically modified bacteria to deliver molecules of therapeutic interest at mucosal surfaces. Due to the well-recognized probiotic properties of some strains, bifidobacteria represent excellent candidates for the development of live vehicles to produce and deliver heterologous proteins at mucosal surfaces. However, very few studies have considered this genus because of its complexity to be genetically manipulated. In this work, we report the development of a new Bifidobacteria Expression SysTem (BEST) allowing the production of heterologous proteins in Bifidobacterium bifidum. This system is based on: i) the broad host range plasmid pWV01, ii) a stress-inducible promoter, and iii) two different signal peptides (SPs) one issued from Lactococcus lactis (SPExp4) and issued from Bifidobacterium longum (SPBL1181). The functionality of BEST system was validated by cloning murine interleukin-10 (IL-10) and establishing the resulting plasmids (i.e., pBEST(Exp4):IL-10 and pBEST(BL1181):IL-10) in the strain of B. bifidum BS42. We then demonstrated in vitro that recombinant B. bifidum BS42 harboring pBEST(BL1181):IL-10 plasmid efficiently secreted IL-10 and that this secretion was significantly higher (sevenfold) than its counterpart B. bifidum BS42 harboring pBEST(Exp4):IL-10 plasmid. Finally, we validated in vivo that recombinant B. bifidum strains producing IL-10 using BEST system efficiently delivered this cytokine at mucosal surfaces and exhibit beneficial effects in a murine model of low-grade intestinal inflammation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/30622516; PRODINRA: 468698; PUBMED: 30622516; WOS: 000454068100001 |
| DOI: | 10.3389/fmicb.2018.03075 |
| الاتاحة: | https://cnrs.hal.science/hal-02126568 https://cnrs.hal.science/hal-02126568v1/document https://cnrs.hal.science/hal-02126568v1/file/2018_Mauras_Frontiers%20in%20Microbiology_1.pdf https://doi.org/10.3389/fmicb.2018.03075 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C5F74177 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2018.03075 |
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