Dissertation/ Thesis
Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats ; Učinci novih perfluorokarbonskih nanočestica stvorenih pomoću albumina u liječenju plinskih embolizama uzrokovanih dekompresijom u štakora
| العنوان: | Effects of newly designed albumin-derived perfluorocarbon-based nanoparticles in the therapy of decompression induced gas embolisms in rats ; Učinci novih perfluorokarbonskih nanočestica stvorenih pomoću albumina u liječenju plinskih embolizama uzrokovanih dekompresijom u štakora |
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| المؤلفون: | Mayer, Dirk |
| المساهمون: | Ferenz, Katja Bettina |
| بيانات النشر: | Sveučilište u Splitu. Medicinski fakultet. University of Split. School of Medicine. |
| سنة النشر: | 2023 |
| المجموعة: | The University of Split Repository |
| مصطلحات موضوعية: | BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti. Fiziologija čovjeka, BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences. Human Physiology, Patologija. Klinička medicina, Pathology. Clinical medicine, info:eu-repo/classification/udc/616(043.3), Air Embolism, Decompression Sickness, Nanocapsules, Fluorocarbons, Wistar Rats, albumin-derived perfluorocarbon-based artificial oxygen carriers, plinska embolija, dekompresijska bolest, nanokapsule, fluorougljici, Wistar štakori, umjetni nosači kisika na bazi perfluorougljika dobiveni iz albumina |
| الوصف: | For three decades, studies have attempted to demonstrate the therapeutic efficacy of perfluorocarbons (PFCs) in reducing the onset of decompression trauma. However, none of these emulsion-based preparations are accepted for therapeutic use in the western world, mainly because of severe side-effects and a long organ retention time. A new development to guarantee a stable dispersion without these disadvantages is the encapsulation of PFCs in nanocapsules with an albumin shell. Purpose: Newly designed albumin-derived perfluorocarbon-based artificial oxygen carriers (AAOCs) are used in a rodent in-vivo model as a preventive therapy for decompression sickness (DCS). Methods: Thirty-seven rats were treated with either A-AOCs (n=12), albumin nanocapsules filled with neutral oil (A-O-N, n=12) or 5% human serum albumin solution (A-0-0, n=13) before a simulated dive. Eleven rats, injected with A-AOCs, stayed at normal pressure (A-AOCssurface). Clinical, laboratory and histological evaluations were performed. Results: The occurrence of DCS depended on the treatment group. A-AOCs significantly reduced DCS-appearance and mortality. Furthermore, a significant improvement of survival rate was found (A-AOCs compared with A-0-0). Histological assessment of A-AOCs-dive compared with A-0-0-dive animals revealed significantly higher accumulation of macrophages, but less blood congestion in the spleen and significantly less hepatic circulatory disturbance, vacuolisation, and cell damage. Compared to non-diving controls lactate and myoglobin showed a significant increase in the A-0-0- but not in the A-AOCs-dive group. Conclusion: Intravenous application of A-AOCs was well tolerated and effective in reducing the occurrence of DCS, animals showed significantly higher survival rates and less symptoms compared to the albumin group (A-0-0). Analysis of histological results and fast reacting plasma parameters confirmed the preventive properties of A-AOCs. ; Istraživanja kojima se ispituje moguća terapijska učinkovitost perfluorokarbona ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://repozitorij.svkst.unist.hr/islandora/object/mefst:2197; https://urn.nsk.hr/urn:nbn:hr:171:644311; https://repozitorij.svkst.unist.hr/islandora/object/mefst:2197/datastream/PDF |
| الاتاحة: | https://repozitorij.svkst.unist.hr/islandora/object/mefst:2197 https://urn.nsk.hr/urn:nbn:hr:171:644311 https://repozitorij.svkst.unist.hr/islandora/object/mefst:2197/datastream/PDF |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C596DD7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |