Academic Journal
Prestress and adhesion site dynamics control cell sensitivity to extracellular stiffness. ; : Extracellular stiffness cell sensitivity
| العنوان: | Prestress and adhesion site dynamics control cell sensitivity to extracellular stiffness. ; : Extracellular stiffness cell sensitivity |
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| المؤلفون: | Féréol, Sophie, Fodil, Redouane, Laurent, Valérie, M., Balland, Martial, Louis, Bruno, Pelle, Gabriel, Hénon, Sylvie, Planus, Emmanuelle, Isabey, Daniel |
| المساهمون: | INSERM U955, équipe 13, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire Interdisciplinaire de Physique Saint Martin d’Hères (LIPhy), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut Mondor de Recherche Biomédicale (IMRB), Matière et Systèmes Complexes (MSC), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Service de physiologie, explorations fonctionnelles Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut Mondor de Recherche Biomédicale (IMRB), The authors are grateful to the Région d'Ile-de-France for financial support for the AFM equipment acquired by University Paris XII. |
| المصدر: | ISSN: 0006-3495. |
| بيانات النشر: | CCSD Biophysical Society |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Dissociation force, Cell mechanosensitivity, Cell mechanics, Force regulation by adhesion sites, Extracellular Environment, Substrate rigidity, MESH: Actomyosin, MESH: Animals, MESH: Extracellular Space, MESH: Humans, MESH: Macrophages, Alveolar, MESH: Magnetics, MESH: Models, Biological, MESH: Pulmonary Alveoli, MESH: Rats, Sprague-Dawley, MESH: Respiratory Mucosa, MESH: Torque, MESH: Cell Adhesion, MESH: Cell Line, MESH: Cells, Cultured, MESH: Collagen Type I, MESH: Computer Simulation, MESH: Cytochalasin D, MESH: Elastic Modulus, MESH: Epithelial Cells, [SDV.BBM]Life Sciences [q-bio]/Biochemistry |
| الوصف: | International audience ; This study aims at improving the understanding of mechanisms responsible for cell sensitivity to extracellular environment. We explain how substrate mechanical properties can modulate the force regulation of cell sensitive elements primarily adhesion sites. We present a theoretical and experimental comparison between two radically different approaches of the force regulation of adhesion sites that depends on their either stationary or dynamic behavior. The most classical stationary model fails to predict cell sensitivity to substrate stiffness whereas the dynamic model predicts extracellular stiffness dependence. This is due to a time dependent reaction force in response to actomyosin traction force exerted on cell sensitive elements. We purposely used two cellular models, i.e., alveolar epithelial cells and alveolar macrophages exhibiting respectively stationary and dynamic adhesion sites, and compared their sensitivity to theoretical predictions. Mechanical and structural results show that alveolar epithelial cells exhibit significant prestress supported by evident stress fibers and lacks sensitivity to substrate stiffness. On the other hand, alveolar macrophages exhibit low prestress and exhibit sensitivity to substrate stiffness. Altogether, theory and experiments consistently show that adhesion site dynamics and cytoskeleton prestress control cell sensitivity to extracellular environment with an optimal sensitivity expected in the intermediate range. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19254561; PUBMED: 19254561 |
| DOI: | 10.1016/j.bpj.2008.10.072 |
| الاتاحة: | https://inserm.hal.science/inserm-00371938 https://inserm.hal.science/inserm-00371938v1/document https://inserm.hal.science/inserm-00371938v1/file/inserm-00371938_edited.pdf https://inserm.hal.science/inserm-00371938v1/file/FEREOL_ET_AL_BIOPHYSJ_2008_143180_TEXT_FIGURES_FINAL_POUR_PUBLICATION.pdf https://doi.org/10.1016/j.bpj.2008.10.072 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C590B242 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.bpj.2008.10.072 |
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