Academic Journal
Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa
| العنوان: | Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa |
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| المؤلفون: | Lan, Nan, Ye, Shuting, Hu, Chengcheng, Chen, Zhiling, Huang, Jun, Xue, Wei, Li, Shaojie, Sun, Xianyun |
| المصدر: | Frontiers in Microbiology ; volume 12 ; ISSN 1664-302X |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2021 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Mitogen-activated protein (MAP) kinase pathways function as signaling hubs that are integral for many essential cellular processes, including sexual development. The molecular mechanisms and cross-talk between PR and CWI MAP kinase pathways have been extensively studied during asexual development. However, if these can be extended to sexual development remains elusive. By analyzing genome-wide transcriptional responses to deletion of each of two MAP kinase coding genes mak - 2 (PR-MAP kinase pathway) and mak - 1 (CWI-MAP kinase pathway) in Neurospora crassa during protoperithecium formation, 430 genes co-regulated by the MAK-1 and MAK-2 proteins were found, functionally enriched at integral components of membrane and oxidoreductase. These genes include 13 functionally known genes participating in sexual development ( app , poi-2 , stk-17 , fsd-1 , vsd-8 , and NCU03863) and melanin synthesis ( per-1 , pkh-1 , pkh-2 , mld-1 , scy-1 , trn-2 , and trn-1 ), as well as a set of functionally unknown genes. Phenotypic analysis of deletion mutants for the functionally unknown genes revealed that 12 genes were essential for female fertility. Among them, single-gene deletion mutants for NCU07743 (named as pfd-1 ), NCU02250 ( oli ), and NCU05948 (named as pfd-2 ) displayed similar protoperithecium development defects as the Δ mak - 1 and Δ mak - 2 mutants, failing to form protoperithecium. Western blotting analysis showed that both phosphorylated and total MAK-1 proteins were virtually abolished in the Δ nrc - 1 , Δ mek - 2 , and Δ mak - 2 mutants, suggesting that the posttranscriptional regulation of MAK-1 is dependent on the PR-MAP kinase pathway during the protoperithecium development. Taken together, this study revealed the regulatory roles and cross-talk between PR and CWI-MAP kinase pathways during protoperithecium development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fmicb.2021.769615 |
| DOI: | 10.3389/fmicb.2021.769615/full |
| الاتاحة: | http://dx.doi.org/10.3389/fmicb.2021.769615 https://www.frontiersin.org/articles/10.3389/fmicb.2021.769615/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.C4D42AAB |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2021.769615 |
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