Academic Journal
Cinnamic acid and lactobionic acid based nanoformulations as a potential antiamoebic therapeutics
| العنوان: | Cinnamic acid and lactobionic acid based nanoformulations as a potential antiamoebic therapeutics |
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| المؤلفون: | Akbar, Noor, Kawish, Muhammad, Jabri, Tooba, Khan, Naveed Ahmed, Shah, Muhammad Raza, Siddiqui, Ruqaiyyah |
| بيانات النشر: | Academic Press Inc Elsevier Science |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Acanthamoeba Castellanii, Magnetic Nanoparticles, Hesperidin, Amphotericin B, Encystation, Excystation, Cytotoxicity, Cytopathogenicity |
| الوصف: | Acanthamoeba castellanii causes granulomatous amoebic encephalitis, an uncommon but severe brain infection and sight-threatening Acanthamoeba keratitis. Most of the currently used anti-amoebic treatments are not always effective, due to persistence of the cyst stage, and recurrence can occur. Here in this study we synthesize cin-namic acid and lactobionic acid-based magnetic nanoparticles (MNPs) using co-precipitation technique. These nanoformulations were characterized by Fourier transform infrared spectroscopy and Atomic form microscopy. The drugs alone (Hesperidin, Curcumin and Amphotericin B), magnetic NPs alone, and drug-loaded nano -for-mulations were evaluated at a concentration of 100 mu g/mL for antiamoebic activity against a clinical isolate of A. castellanii. Amoebicidal assays revealed that drugs and conjugation of drugs and NPs further enhanced amoebicidal effects of drug-loaded nanoformulations. Drugs and drug-loaded nanoformulations inhibited both encystation and excystation of amoebae. In addition, drugs and drug-loaded nanoformulations inhibited parasite binding capability to the host cells. Neither drugs nor drug-loaded nanoformulations showed cytotoxic effects against host cells and considerably reduced parasite-mediated host cell death. Overall, these findings imply that conjugation of medically approved drugs with MNPs produce potent anti-Acanthamoebic effects, which could eventually lead to the development of therapeutic medications. ; American University of Sharjah and the University of Sharjah ; Authors acknowledge the American University of Sharjah and the University of Sharjah for their support. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0014-4894 1090-2449 |
| Relation: | Experimental Parasitology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1016/j.exppara.2023.108474; https://hdl.handle.net/20.500.12713/4601; 246; WOS:000963596400001; 2-s2.0-85147294559; N/A; Q2 |
| DOI: | 10.1016/j.exppara.2023.108474 |
| الاتاحة: | https://hdl.handle.net/20.500.12713/4601 https://doi.org/10.1016/j.exppara.2023.108474 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.C4CE0839 |
| قاعدة البيانات: | BASE |
| تدمد: | 00144894 10902449 |
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| DOI: | 10.1016/j.exppara.2023.108474 |