Academic Journal
EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex
| العنوان: | EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex |
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| المؤلفون: | Liu, Guangqiao, Song, Guangtao, Zhang, Danyang, Zhang, Dejiu, Li, Zhikai, Lyu, Zhixin, Dong, Jianshu, Achenbach, John, Gong, Weimin, Zhao, Xin Sheng, Nierhaus, Knud H., Qin, Yan |
| المساهمون: | Qin, Y (reprint author), Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing 100080, Peoples R China., Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing 100080, Peoples R China., Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China., Peking Univ, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China., NOXXON Pharma AG, Berlin, Germany., Charite, Inst Med Phys & Biophys, D-13353 Berlin, Germany. |
| المصدر: | SCI |
| بيانات النشر: | 自然结构与分子生物学 |
| سنة النشر: | 2014 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | ELONGATION-FACTOR-G, PROTECTION PROTEIN TET(O), 30S RIBOSOMAL-SUBUNIT, MESSENGER-RNA, TETRACYCLINE RESISTANCE, PRETRANSLOCATION STATE, INTERMEDIATE STATES, ESCHERICHIA-COLI, STEM-LOOP, P-SITES |
| الوصف: | During translation, elongation factor G (EF-G) catalyzes the translocation of tRNA(2)-mRNA inside the ribosome. Trans location is coupled to a cycle of conformational rearrangements of the ribosomal machinery, and how EF-G initiates translocation remains unresolved. Here we performed systematic mutagenesis of Escherichia coli EF-G and analyzed inhibitory single-site mutants of EF-G that preserved pretranslocation (Pre)-state ribosomes with tRNAs in A/P and PIE sites (Pre-EF-G). Our results suggest that the interactions between the decoding center and the codon-anticodon duplex constitute the barrier for translocation. Catalysis of translocation by EF-G involves the factor's highly conserved loops I and II at the tip of domain IV, which disrupt the hydrogen bonds between the decoding center and the duplex to release the latter, hence inducing subsequent translocation events, namely 30S head swiveling and tRNA(2)-mRNA movement on the 30S subunit. ; Biochemistry & Molecular Biology ; Biophysics ; Cell Biology ; SCI(E) ; 0 ; ARTICLE ; qiny@ibp.ac.cn ; 9 ; 817-824 ; 21 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1545-9993 1545-9985 |
| Relation: | NATURE STRUCTURAL & MOLECULAR BIOLOGY.2014,21,(9),817-824.; 763792; http://hdl.handle.net/20.500.11897/388336; WOS:000341404200012 |
| DOI: | 10.1038/nsmb.2869 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/388336 https://doi.org/10.1038/nsmb.2869 |
| رقم الانضمام: | edsbas.C4B85BD2 |
| قاعدة البيانات: | BASE |
| تدمد: | 15459993 15459985 |
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| DOI: | 10.1038/nsmb.2869 |