Academic Journal
INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.)
| العنوان: | INDUKSIKALUS DAN REGENERASI TUNAS PULAI PANDAK (Rauwolfta serpentina L.) |
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| المؤلفون: | Yunita, Rossa, Lestari, Endang Gati |
| المصدر: | BERITA BIOLOGI; Vol 9, No 1 (2008); 91 – 97 ; 2337-8751 ; 0126-1754 |
| بيانات النشر: | Research Center for Biology-Indonesian Institute of Sciences |
| سنة النشر: | 2016 |
| المجموعة: | E-Journal Portal - Research Center for Biology - Indonesian Institute of Sciences (LIPI) / Pusat Penelitian Biologi - Lembaga Ilmu Pengetahuan Indonesia |
| مصطلحات موضوعية: | Induksi kalus, regenerasi, pulai pandak, Rauwolfta serpentina |
| الوصف: | In vitro culture can be applied for producing new genotype which is tolerant to biotic and abiotic or to incerase secondary metabolic content. To obtain the optimum result of variety improvement, regeneration system should firstly be found out.It is sufficiently difficult to regenerate pulai pandak (Rauwolfia serpentina L.). Hence, with this system, the improvement of R. serpentina with secondary metabolic content higher than the other. The mother stok of R. serpentina used in this experiment, belongs to the collection of BB-Biogen. Calli were produced from leaves and internodes which is cultured at medium MS contain 2.4-D (0, 1, 3,5, 7 mg/1) combined with caseine hydrolysate 3 mg/1. Regeneration medium was MS contain BA (0,5, 1 mg/1) combined with zeatin (0, 0.1 and 0.5 mg/1) and root formation used was three kinds of auxin (IBA, IAA and NAA). The result showed that inter nodels was better that leaves to callus induction. In this experiment, MS + 2,4-D 1 mg/1 + CH 3 mg/1 was the best medium to induct calli,while medium MS + BA 1 mg/1 + Zeatin 0,5 mg/1 + maltosa 3% to regenerate and MS + IBA lmg/1 for root induction. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/807/579; https://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/807 |
| DOI: | 10.14203/beritabiologi.v9i1.807 |
| الاتاحة: | https://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/807 https://doi.org/10.14203/beritabiologi.v9i1.807 |
| Rights: | Copyright (c) 2015 BERITA BIOLOGI |
| رقم الانضمام: | edsbas.C3EF67A1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.14203/beritabiologi.v9i1.807 |
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