التفاصيل البيبلوغرافية
| العنوان: |
Synaptic removal of diacylglycerol by DGKζ and PSD-95 regulates dendritic spine maintenance |
| المؤلفون: |
Kim, Karam, Yang, Jinhee, Zhong, Xiao-Ping, Kim, Myoung-Hwan, Kim, Yun Sook, Lee, Hyun Woo, Han, Seungnam, Choi, Jeonghoon, Han, Kihoon, Seo, Jinsoo, Prescott, Stephen M, Topham, Matthew K, Bae, Yong Chul, Koretzky, Gary, Choi, Se-Young, Kim, Eunjoon |
| المساهمون: |
김가람, 양진희, 김명환, 김연숙, 이현우, 한승남, 최정훈, 한기훈, 서진수, 배용철, 최세영, 김은준 |
| بيانات النشر: |
Nature Publishing Group |
| سنة النشر: |
2009 |
| المجموعة: |
Seoul National University: S-Space |
| مصطلحات موضوعية: |
DGKζ, diacylglycerol kinase, phosphatidic acid, PSD-95, spine |
| الوصف: |
Diacylglycerol (DAG) is an important lipid signalling molecule that exerts an effect on various effector proteins including protein kinase C. A main mechanism for DAG removal is to convert it to phosphatidic acid (PA) by DAG kinases (DGKs). However, it is not well understood how DGKs are targeted to specific subcellular sites and tightly regulates DAG levels. The neuronal synapse is a prominent site of DAG production. Here, we show that DGKζ is targeted to excitatory synapses through its direct interaction with the postsynaptic PDZ scaffold PSD-95. Overexpression of DGKζ in cultured neurons increases the number of dendritic spines, which receive the majority of excitatory synaptic inputs, in a manner requiring its catalytic activity and PSD-95 binding. Conversely, DGKζ knockdown reduces spine density. Mice deficient in DGKζ expression show reduced spine density and excitatory synaptic transmission. Time-lapse imaging indicates that DGKζ is required for spine maintenance but not formation. We propose that PSD-95 targets DGKζ to synaptic DAG-producing receptors to tightly couple synaptic DAG production to its conversion to PA for the maintenance of spine density. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
0261-4189 |
| Relation: |
The EMBO Journal 28:1170-1179; http://hdl.handle.net/10371/74034 |
| الاتاحة: |
http://hdl.handle.net/10371/74034 |
| رقم الانضمام: |
edsbas.C3C73DDD |
| قاعدة البيانات: |
BASE |
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