Academic Journal
Induction of apoptosis by gluconasturtiin-isothiocyanate (GNST-ITC) in human hepatocarcinoma HepG2 cells and human breast adenocarcinoma MCF-7 cells
| العنوان: | Induction of apoptosis by gluconasturtiin-isothiocyanate (GNST-ITC) in human hepatocarcinoma HepG2 cells and human breast adenocarcinoma MCF-7 cells |
|---|---|
| المؤلفون: | Arumugam, Asvinidevi, Ibrahim, Muhammad Din, Kntayya, Saie Brindha, Mohd Ain, Nooraini, Iori, Renato, Galletti, Stefania, Ioannides, Costas, Abdull Razis, Ahmad Faizal |
| بيانات النشر: | MDPI |
| سنة النشر: | 2020 |
| المجموعة: | Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository |
| الوصف: | Gluconasturtiin, a glucosinolate present in watercress, is hydrolysed by myrosinase to form gluconasturtiin-isothiocyanate (GNST-ITC), which has potential chemopreventive effects; however, the underlying mechanisms of action have not been explored, mainly in human cell lines. The purpose of the study is to evaluate the cytotoxicity of GNST-ITC and to further assess its potential to induce apoptosis. GNST-ITC inhibited cell proliferation in both human hepatocarcinoma (HepG2) and human breast adenocarcinoma (MCF-7) cells with IC50 values of 7.83 µM and 5.02 µM, respectively. Morphological changes as a result of GNST-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GNST-ITC in a time-dependent manner. To delineate the mechanism of apoptosis, cell cycle arrest and expression of caspases were studied. GNST-ITC induced a time-dependent G2/M phase arrest, with reduction of 82% and 93% in HepG2 and MCF-7 cell lines, respectively. The same treatment also led to the subsequent expression of caspase-3/7 and -9 in both cells demonstrating mitochondrial-associated cell death. Collectively, these results reveal that GNST-ITC can inhibit cell proliferation and can induce cell death in HepG2 and MCF-7 cancer cells via apoptosis, highlighting its potential development as an anticancer agent. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | http://psasir.upm.edu.my/id/eprint/38128/1/38128.pdf; Arumugam, Asvinidevi and Ibrahim, Muhammad Din and Kntayya, Saie Brindha and Mohd Ain, Nooraini and Iori, Renato and Galletti, Stefania and Ioannides, Costas and Abdull Razis, Ahmad Faizal (2020) Induction of apoptosis by gluconasturtiin-isothiocyanate (GNST-ITC) in human hepatocarcinoma HepG2 cells and human breast adenocarcinoma MCF-7 cells. Molecules, 25 (5). art. no. 1240. pp. 1-16. ISSN 1420-3049 |
| الاتاحة: | http://psasir.upm.edu.my/id/eprint/38128/ http://psasir.upm.edu.my/id/eprint/38128/1/38128.pdf https://www.mdpi.com/1420-3049/25/5/1240 |
| رقم الانضمام: | edsbas.C3A77AB8 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |