التفاصيل البيبلوغرافية
| العنوان: |
Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator |
| المؤلفون: |
Bannert, Helmut, Muranyi, Walter, Ogryzko, Vasily V, Nakatani, Yoshihiro, Flügel, Rolf M |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2004 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background Foamy virus Bel1/Tas trans -activators act as key regulators of gene expression and directly bind to Bel1 response elements (BRE) in both the internal and the 5'LTR promoters leading to strong transcriptional trans -activation. Cellular coactivators interacting with Bel1/Tas are unknown to date. Results Transient expression assays, co-immunoprecipitation experiments, pull-down assays, and Western blot analysis were used to demonstrate that the coactivator p300 and histone acetyltransferase PCAF specifically interact with the retroviral trans -activator Bel1/Tas in vivo . Here we show that the Bel1/Tas-mediated trans -activation was enhanced by the coactivator p300, histone acetyltransferases PCAF and SRC-1 based on the crucial internal promoter BRE. The Bel1/Tas-interacting region was mapped to the C/H1 domain of p300 by co-immunoprecipitation and pull-down assays. In contrast, coactivator SRC-1 previously reported to bind to the C-terminal domain of p300 did not directly interact with the Bel1 protein but nevertheless enhanced Bel1/Tas-mediated trans -activation. Cotransfection of Bel1/Tas and p300C with an expression plasmid containing the C/H1domain partially inhibited the p300C-driven trans -activation. Conclusions Our data identify p300 and PCAF as functional partner molecules that directly interact with Bel1/Tas. Since the acetylation activities of the three coactivators reside in or bind to the C-terminal regions of p300, a C/H1 expression plasmid was used as inhibitor. This is the first report of a C/H1 domain-interacting retroviral trans -activator capable of partially blocking the strong Bel1/Tas-mediated activation of the C-terminal region of coactivator p300. The potential mechanisms and functional roles of the three histone and factor acetyltransferases p300, PCAF, and SRC-1 in Bel1/Tas-mediated trans -activation are discussed. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
http://www.biomedcentral.com/1471-2199/5/16 |
| الاتاحة: |
http://www.biomedcentral.com/1471-2199/5/16 |
| Rights: |
Copyright 2004 Bannert et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.C366B441 |
| قاعدة البيانات: |
BASE |