Academic Journal
Glycan‐Mediated, Ligand‐Controlled Click Chemistry for Drug‐Target Identification
| العنوان: | Glycan‐Mediated, Ligand‐Controlled Click Chemistry for Drug‐Target Identification |
|---|---|
| المؤلفون: | Stöckmann, Henning, Marin, Violeta L., Nimmer, Paul, Balut, Corina M., Davidson, Donald J., Richardson, Paul L., Vasudevan, Anil |
| المصدر: | ChemBioChem ; volume 17, issue 2, page 150-154 ; ISSN 1439-4227 1439-7633 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Membrane‐bound proteins are important pharmaceutical drug targets, yet few strategies exist for the identification of small‐molecule‐targeted membrane proteins in live‐cell systems. By exploiting metabolic glycan engineering of cell membrane proteins, we have developed an in situ glycan‐mediated ligand‐controlled click (“GLiCo‐Click”) chemistry methodology that enables the attachment of small‐molecule chemical probes to their receptor protein through glycans on live cells. In addition to enabling receptor enrichment from cell lysates, this strategy can be used to demonstrate target receptor engagement and enables the molecular characterization of receptors. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/cbic.201500590 |
| الاتاحة: | http://dx.doi.org/10.1002/cbic.201500590 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcbic.201500590 https://onlinelibrary.wiley.com/doi/full/10.1002/cbic.201500590 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.C34A3B9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/cbic.201500590 |
|---|