Academic Journal
Non-specific interference of cobalt with siderophore-dependent iron uptake pathways
| العنوان: | Non-specific interference of cobalt with siderophore-dependent iron uptake pathways |
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| المؤلفون: | Carballido Lopez, Ana, Cunrath, Olivier, Forster, Anne, Pérard, Julien, Graulier, Gwenaëlle, Legendre, Rachel, Varet, Hugo, Sismeiro, Odile, Perraud, Quentin, Pesset, Bénédicte, Saint Auguste, Pamela, Bumann, Dirk, Mislin, Gaetan, Coppee, Jean Yves, Michaud-Soret, Isabelle, Fechter, Pierre, Schalk, Isabelle |
| المساهمون: | Biotechnologie et signalisation cellulaire (BSC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Transcriptome et Epigénome (PF2), Institut Pasteur Paris (IP), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Biozentrum Basel, Suisse, Université de Bâle = University of Basel = Basel Universität (Unibas), This work was partly funded by the Centre National de la Recherche Scientifique. O. Cunrath held a fellowship from the French Ministère de la Recherche et de la Technologie (3 years) and the FRM (Fondation pour la Recherche Médicale, 6 months). A. Y. Carballido Lopez held a fellowship from the University of Strasbourg (IDEX) and B. Pesset from the DGA (Direction Générale des l’Armement). J. P. and I. M. S. thank the Laboratory of Excellence GRAL (grant ANR-11-LABX-49-01) and the Labex ARCANE (grant ANR-11-LABX-0003-01). The Transcriptome and Epigenome Platform is a member of the France Génomique consortium (ANR10-NBS-09-08)., We thank Dr A. Boos and P. Ronot (Institut Pluridisciplinaire Hubert Curien, Strasbourg) for their help in the ICP-AES measurements., Labex Arcane, ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), ANR-11-LABX-0049,EHNE,Écrire une histoire nouvelle de l'Europe(2011), ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010) |
| المصدر: | ISSN: 1756-5901. |
| بيانات النشر: | CCSD Royal Society of Chemistry |
| سنة النشر: | 2019 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | Much data shows that biological metals other than Fe3+ can interfere with Fe3+ acquisition by siderophores in bacteria. Siderophores are small Fe3+ chelators produced by the microorganisms to obtain access to Fe3+. Here, we show that Co2+ is imported into Pseudomonas aeruginosa cells in a complex with the siderophore pyochelin (PCH) by the ferri-PCH outer membrane transporter FptA. Moreover, the presence of Co2+ in the bacterial environment strongly affects the production of PCH. Proteomic and transcriptomic approaches showed that a decrease of PCH production is associated with repression of the expression of the genes involved in PCH biosynthesis. We used various molecular biology approaches to show that this repression is not Fur-(ferric uptake transcriptional regulator) dependent but due to competition of PCH-Co with PCH-Fe for PchR (transcriptional activator), thus inhibiting the formation of PchR-PCH-Fe and consequently the expression of the PCH genes. We observed a similar mechanism of repression of PCH production, but to a lesser extent, by Ni2+, but not for Zn2+, Cu2+, or Mn2+. Here, we show, for the first time at a molecular level, how the presence of a contaminant metal can interfere with Fe3+ acquisition by the siderophores PCH and PVD. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1039/C9MT00195F |
| الاتاحة: | https://hal.science/hal-02955479 https://hal.science/hal-02955479v1/document https://hal.science/hal-02955479v1/file/PDF%20Datastream-15-1.pdf https://doi.org/10.1039/C9MT00195F |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C3313CD5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/C9MT00195F |
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