Academic Journal
Detection by hemi-nested reverse transcription polymerase chain reaction and genetic characterization of wild type strains of Canine distemper virus in suspected infected dogs
| العنوان: | Detection by hemi-nested reverse transcription polymerase chain reaction and genetic characterization of wild type strains of Canine distemper virus in suspected infected dogs |
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| المؤلفون: | Di Francesco, Cristina E., Di Francesco, Daniela, Di Martino, Barbara, Speranza, Roberto, Santori, Domenico, Boari, Andrea, Marsilio, Fulvio |
| المصدر: | Journal of Veterinary Diagnostic Investigation ; volume 24, issue 1, page 107-115 ; ISSN 1040-6387 1943-4936 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2011 |
| الوصف: | A new highly sensitive and specific hemi-nested reverse transcription polymerase chain reaction (RT-PCR) assay was applied to detect nucleoprotein ( NP) gene of Canine distemper virus (CDV) in samples collected from dogs showing respiratory, gastrointestinal, and neurological signs. Thirty-eight out of 86 samples were positive suggesting that despite the vaccination, canine distemper may still represent a high risk to the canine population. The 968 base pair (bp) fragments from the hemagglutinin ( H) gene of 10 viral strains detected in positive samples were amplified and analyzed by restriction fragment length polymorphism (RFLP) using AluI and PsiI enzymes in order to differentiate among vaccine and wild-type CDV strains and to characterize the field viral strains. The products of the both enzymatic digestions allowed identification all viruses as wild strains of CDV. In addition, the RFLP analysis with AluI provided additional information about the identity level among the strains analyzed on the basis of the positions of the cleavage site in the nucleotide sequences of the H gene. The method could be a more useful and simpler method for molecular studies of CDV strains. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/1040638711425700 |
| الاتاحة: | http://dx.doi.org/10.1177/1040638711425700 http://journals.sagepub.com/doi/pdf/10.1177/1040638711425700 http://journals.sagepub.com/doi/full-xml/10.1177/1040638711425700 |
| Rights: | http://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.C3208CEF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/1040638711425700 |
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