Academic Journal
Detection and isolation of airborne SARS-CoV-2 in a hospital setting
| العنوان: | Detection and isolation of airborne SARS-CoV-2 in a hospital setting |
|---|---|
| المؤلفون: | de Sousa, Nuno Rufino, Steponaviciute, Laura, Margerie, Lucille, Nissen, Karolina, Kjellin, Midori, Reinius, Bjorn, Salaneck, Erik, Udekwu, Klas, Rothfuchs, Antonio Gigliotti |
| سنة النشر: | 2022 |
| المجموعة: | Swedish University of Agricultural Sciences / Sveriges Lantbruksuniversitet (SLU): Epsilon Open Archive |
| مصطلحات موضوعية: | Environmental Health and Occupational Health |
| الوصف: | Transmission mechanisms for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are incompletely understood. In particular, aerosol transmission remains unclear, with viral detection in air and demonstration of its infection potential being actively investigated. To this end, we employed a novel electrostatic collector to sample air from rooms occupied by COVID-19 patients in a major Swedish hospital. Electrostatic air sampling in conjunction with extraction-free, reverse-transcriptase polymerase chain reaction (hid-RT-PCR) enabled detection of SARS-CoV-2 in air from patient rooms (9/22; 41%) and adjoining anterooms (10/22; 45%). Detection with hid-RT-PCR was concomitant with viral RNA presence on the surface of exhaust ventilation channels in patients and anterooms more than 2 m from the COVID-19 patient. Importantly, it was possible to detect active SARS-CoV-2 particles from room air, with a total of 496 plaque-forming units (PFUs) being isolated, establishing the presence of infectious, airborne SARS-CoV-2 in rooms occupied by COVID-19 patients. Our results support circulation of SARS-CoV-2 via aerosols and urge the revision of existing infection control frameworks to include airborne transmission. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://pub.epsilon.slu.se/27606/1/de-sousa-n-r-et-al-220425.pdf; de Sousa, Nuno Rufino and Steponaviciute, Laura and Margerie, Lucille and Nissen, Karolina and Kjellin, Midori and Reinius, Bjorn and Salaneck, Erik and Udekwu, Klas and Rothfuchs, Antonio Gigliotti (2022). Detection and isolation of airborne SARS-CoV-2 in a hospital setting. Indoor Air. 32 :3 , e13023 [Research article] |
| الاتاحة: | https://pub.epsilon.slu.se/27606/ https://pub.epsilon.slu.se/27606/1/de-sousa-n-r-et-al-220425.pdf |
| رقم الانضمام: | edsbas.C30B3770 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |