Academic Journal
In silico and in vitro analysis of lncRNA XIST reveals a panel of possible lung cancer regulators and a five-gene diagnostic signature
| العنوان: | In silico and in vitro analysis of lncRNA XIST reveals a panel of possible lung cancer regulators and a five-gene diagnostic signature |
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| المؤلفون: | Katopodis, P, Dong, Q, Halai, H, Fratila, CI, Polychronis, A, Anikin, V, Sisu, C, Karteris, E |
| بيانات النشر: | MDPI |
| سنة النشر: | 2020 |
| المجموعة: | Brunel University London: Brunel University Research Archive (BURA) |
| مصطلحات موضوعية: | XIST, x-inactivation center, NSCLC, lncRNA, TCGA, GTEX, RNA-seq, lung cancer, bioinformatics |
| الوصف: | © 2020 by the authors. Long non-coding RNAs (lncRNAs) perform a wide functional repertoire of roles in cell biology, ranging from RNA editing to gene regulation, as well as tumour genesis and tumour progression. The lncRNA X-inactive specific transcript (XIST) is involved in the aetiopathogenesis of non-small cell lung cancer (NSCLC). However, its role at the molecular level is not fully elucidated. The expression of XIST and co-regulated genes TSIX, hnRNPu, Bcl-2, and BRCA1 analyses in lung cancer (LC) and controls were performed in silico. Differentially expressed genes (DEGs) were determined using RNA-seq in H1975 and A549 NSCLC cell lines following siRNA for XIST. XIST exhibited sexual dimorphism, being up-regulated in females compared to males in both control and LC patient cohorts. RNA-seq revealed 944 and 751 DEGs for A549 and H1975 cell lines, respectively. These DEGs are involved in signal transduction, cell communication, energy pathways, and nucleic acid metabolism. XIST expression associated with TSIX, hnRNPu, Bcl-2, and BRCA1 provided a strong collective feature to discriminate between controls and LC, implying a diagnostic potential. There is a much more complex role for XIST in lung cancer. Further studies should concentrate on sex-specific changes and investigate the signalling pathways of the DEGs following silencing of this lncRNA. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1 - 16 |
| اللغة: | English |
| تدمد: | 2072-6694 |
| Relation: | Cancers; 3499; Katopodis, P., Dong, Q., Halai, H., Fratila, C. I., Polychronis, A., Anikin, V., Sisu, C. and Karteris, E. (2020) ‘In Silico and In Vitro Analysis of lncRNA XIST Reveals a Panel of Possible Lung Cancer Regulators and a Five-Gene Diagnostic Signature’, Cancers, 12 (12), 3499 , pp. 1 - 16. doi:10.3390/cancers12123499.; https://bura.brunel.ac.uk/handle/2438/23334; https://doi.org/10.3390/cancers12123499 |
| DOI: | 10.3390/cancers12123499 |
| الاتاحة: | https://bura.brunel.ac.uk/handle/2438/23334 https://doi.org/10.3390/cancers12123499 |
| Rights: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.C2EED976 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20726694 |
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| DOI: | 10.3390/cancers12123499 |