Academic Journal
Foodborne Bacterial, Viral, and Protozoan Pathogens in Field and Market Strawberries and Environment of Strawberry Farms
| العنوان: | Foodborne Bacterial, Viral, and Protozoan Pathogens in Field and Market Strawberries and Environment of Strawberry Farms |
|---|---|
| المؤلفون: | Dziedzinska, Radka, Vasickova, Petra, Hrdy, Jakub, Slany, Michal, Babak, Vladimir, Moravkova, Monika |
| المساهمون: | Ministerstvo Školství, Mládeže a Tělovýchovy, Ministerstvo Zemědělství |
| المصدر: | Journal of Food Science ; volume 83, issue 12, page 3069-3075 ; ISSN 0022-1147 1750-3841 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2018 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Field‐grown strawberries, the environment of strawberry farms and fresh strawberries from marketplaces were examined for bacterial, viral, and protozoan pathogens. The presence of bacteria was determined using culture and real‐time PCR (qPCR), presence of protozoa and viruses using qPCR and reverse transcription qPCR, respectively. The highest proportion of positivity was observed for Escherichia coli both in field and purchased strawberries (up to 48.6%). Finding of Cronobacter ranged from 0.6% to 9% both for field and market strawberries. The prevalence of other pathogens ( Listeria monocytogenes , Giardia intestinalis , Cryptosporidium sp., and Norovirus ) in strawberries was below 4.5%; HAV was not detected at all. Positivity of the environment was determined to be lower than 2.1% for all microorganisms, except for E. coli . The concentration of pathogens in most samples did not exceed 100 CFU/g using culture and 1.8 × 10 2 GE/g of strawberries or swabbing area 6.1 × 10 2 GE/mL or swabbing area of environmental samples using qPCR. All studied farms applied preventive measures such as drip irrigation, avoidance of organic fertilizers, and use of mulch foils or gloves for workers to decrease contamination of strawberries. Despite this, certain pathogens were found in fresh strawberries. Even at low concentrations, these pathogens can be a source of infection for consumers. Thus, their presence in strawberries is of particular significance as these are mostly consumed fresh and without any thermal processing. Practical Application Nonlegislatively monitored pathogens of bacterial, viral and parasitic origin were found in strawberries. Monitoring the presence of these pathogens in ready‐to‐eat food is therefore meaningful and important in terms of food safety, especially in relation to pathogens with low infectious dose (for example, viruses, parasites). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/1750-3841.14401 |
| الاتاحة: | http://dx.doi.org/10.1111/1750-3841.14401 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1750-3841.14401 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1750-3841.14401 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1750-3841.14401 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.C2AA7019 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/1750-3841.14401 |
|---|