Academic Journal
Use of standardized protease enzymes for antibody screening of blood donor samples with the microplate system AutoAnalyzer
| العنوان: | Use of standardized protease enzymes for antibody screening of blood donor samples with the microplate system AutoAnalyzer |
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| المؤلفون: | Mazda, T., Makino, K., Yabe, R., Nakata, K., Fujisawa, K., Ohshima, H. |
| المصدر: | Transfusion Medicine ; volume 5, issue 1, page 43-50 ; ISSN 0958-7578 1365-3148 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1995 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | SUMMARY. Papain, bromelin and ficin can be standardized by the casein method for use in red blood cell antibody screening tests. The minimal and optimal enzyme activity for detecting blood group antibodies in donors, using the new Automated Pre‐Transfusion Blood Testing System Olympus PK7200 by the two‐stage method for all three enzymes, is one casein unit. One casein unit of proteinase activity changed the red blood cell surface charge to a low plateau value as measured by electrophoretic mobility and sialic acid content, and removed sterically inhibiting structures of surface protein as detected by SDS‐PAGE. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-3148.1995.tb00184.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1365-3148.1995.tb00184.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3148.1995.tb00184.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-3148.1995.tb00184.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.C278CA0D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-3148.1995.tb00184.x |
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