التفاصيل البيبلوغرافية
| العنوان: |
SC31 binds SARS-CoV-2 SP and neutralizes virus through inhibition of binding to ACE2. |
| المؤلفون: |
Conrad E. Z. Chan (11014434), Shirley G. K. Seah (11014437), De Hoe Chye (11014440), Shane Massey (224366), Maricela Torres (11014443), Angeline P. C. Lim (11014446), Steven K. K. Wong (11014449), Jacklyn J. Y. Neo (11014452), Pui San Wong (11014455), Jie Hui Lim (11014458), Gary S. L. Loh (11014461), Dongling Wang (258597), Jerome D. Boyd-Kirkup (11014464), Siyu Guan (11014467), Dipti Thakkar (11014470), Guo Hui Teo (1521718), Kiren Purushotorman (11014473), Paul E. Hutchinson (6458183), Barnaby E. Young (10869381), Jenny G. Low (10499731), Paul A. MacAry (10534724), Hannes Hentze (6680750), Venkateshan S. Prativadibhayankara (11014476), Kantharaj Ethirajulu (2100358), Jason E. Comer (7908407), Chien-Te K. Tseng (492742), Alan D. T. Barrett (8980565), Piers J. Ingram (11014479), Trevor Brasel (502095), Brendon John Hanson (11014482) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Cell Biology, Molecular Biology, Pharmacology, Biotechnology, Ecology, Immunology, Cancer, Infectious Diseases, Virology, convalescent COVID -19 patient, anti-SARS-CoV -2 activities, SARS-CoV -2-infected hamsters, IFN, K 18-human ACE 2, attenuated pro-inflammatory responses, SARS-CoV -2 Spike protein, efficacy, Fc-null LALA variant, COVID -19. Importantly, Fc-mediated effector functions, SARS-CoV -2 neutralizing antibody, SC 31 treatment, SARS-CoV -2-neutralizing antibodies, SC 31 |
| الوصف: |
( A ) Neutralization of 100 TCID 50 of infectious virus by SC31 IgG1 compared to control IgG1 or IgG purified from donor serum, represented as a percentage relative to uninfected and “virus only” controls. ( B ) Binding affinity of SC31 IgG to purified wild type SP or RBD as measured by ELISA. ( C ) Inhibition of wild-type SP or RBD binding to cells expressing membrane-bound ACE2 by SC31 IgG1 at different concentrations as measured by flow cytometry, expressed as a percentage relative to “no antibody” and “no viral protein” controls. ( D ) Binding affinity of SC31 as determined by ELISA to purified wild-type SP (blue) and SP mutants that either do not affect SC31 or ACE2 binding (green), affect SC31 but not ACE2 binding (purple) or affect both SC31 and ACE2 binding (red). ( E ) Binding affinity of purified wild-type and mutant SP to hACE2-expressing CHO cells based on fluorescence intensity measured by flow cytometry. Statistical significance was determined versus wild-type SP for each mutant by one-way ANOVA: ** p<0.01, *** p<0.001, **** p<0.0001. ( F ). Locations of single amino acid mutations (green/purple/red) on a crystal structure of RBD showing the interaction of the RBM (cyan) with ACE2 N-terminal helix (blue). All results represent the mean of three independent replicates with bars showing standard error. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/SC31_binds_SARS-CoV-2_SP_and_neutralizes_virus_through_inhibition_of_binding_to_ACE2_/14830479 |
| DOI: |
10.1371/journal.pone.0253487.g001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0253487.g001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.C238D857 |
| قاعدة البيانات: |
BASE |