Academic Journal
Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis
| العنوان: | Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis |
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| المؤلفون: | Libraty, Daniel H., Pichyangkul, Sathit, Ajariyakhajorn, Chuanpis, Endy, Timothy P., Ennis, Francis A. |
| المساهمون: | Center for Infectious Disease and Vaccine Research |
| المصدر: | Journal of virology ; 75 ; 8 ; 3501-8 |
| سنة النشر: | 2022 |
| المجموعة: | University of Massachusetts, Medical School: eScholarship@UMMS |
| مصطلحات موضوعية: | Antibodies, Viral, Biological Markers, Cell Differentiation, Cells, Cultured, Dendritic Cells, Dengue Virus, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Interferon Type II, Interferon-alpha, Interleukin-10, Interleukin-12, Myeloid Cells, Neutralization Tests, Tumor Necrosis Factor-alpha, Virus Replication, Life Sciences, Medicine and Health Sciences |
| الوصف: | The ability of dendritic cells (DCs) to shape the adaptive immune response to viral infection is mediated largely by their maturation and activation state as determined by the surface expression of HLA molecules, costimulatory molecules, and cytokine production. Dengue is an emerging arboviral disease where the severity of illness is influenced by the adaptive immune response to the virus. In this report, we have demonstrated that dengue virus infects and replicates in immature human myeloid DCs. Exposure to live dengue virus led to maturation and activation of both the infected and surrounding, uninfected DCs and stimulated production of tumor necrosis factor alpha (TNF-alpha) and alpha interferon (IFN-alpha). Activation of the dengue virus-infected DCs was blunted compared to the surrounding, uninfected DCs, and dengue virus infection induced low-level release of interleukin-12 p70 (IL-12 p70), a key cytokine in the development of cell-mediated immunity (CMI). Upon the addition of IFN-gamma, there was enhanced activation of dengue virus-infected DCs and enhanced dengue virus-induced IL-12 p70 release. The data suggest a model whereby DCs are the early, primary target of dengue virus in natural infection and the vigor of CMI is modulated by the relative presence or absence of IFN-gamma in the microenvironment surrounding the virus-infected DCs. These findings are relevant to understanding the pathogenesis of dengue hemorrhagic fever and the design of new vaccination and therapeutic strategies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Link to Article in PubMed; J Virol. 2001 Apr;75(8):3501-8. Link to article on publisher's site; 0022-538X (Print); http://hdl.handle.net/20.500.14038/38677; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2532&context=oapubs&unstamped=1; https://escholarship.umassmed.edu/oapubs/1533; 798509; oapubs/1533 |
| DOI: | 10.1128/JVI.75.8.3501-3508.2001 |
| الاتاحة: | https://doi.org/10.1128/JVI.75.8.3501-3508.2001 https://hdl.handle.net/20.500.14038/38677 https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2532&context=oapubs&unstamped=1 https://escholarship.umassmed.edu/oapubs/1533 |
| رقم الانضمام: | edsbas.C179EB96 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JVI.75.8.3501-3508.2001 |
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