Academic Journal
Titanium coated with poly(lactic‐co‐glycolic) acid incorporating simvastatin: Biofunctionalization of dental prosthetic abutments
| العنوان: | Titanium coated with poly(lactic‐co‐glycolic) acid incorporating simvastatin: Biofunctionalization of dental prosthetic abutments |
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| المؤلفون: | Littuma, Gustavo J. S., Sordi, Mariane B., Borges Curtarelli, Raissa, Aragonês, Águedo, da Cruz, Ariadne C. C., Magini, Ricardo S. |
| المساهمون: | International Team for Implantology |
| المصدر: | Journal of Periodontal Research ; volume 55, issue 1, page 116-124 ; ISSN 0022-3484 1600-0765 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Objective To propose a biofunctionalized prosthetic abutment by analyzing physico‐chemical and morphological properties, simvastatin (SIM) release, and biocompatibility of titanium (Ti) disks coated with poly(lactic‐co‐glycolic) acid (PLGA) incorporating SIM. Methods Titanium disks (8 × 3 mm) were distributed into four groups: Ti: pure Ti; Ti + PLGA: Ti coated with PLGA; Ti + PLGA + SIM6%: Ti + PLGA with 6% SIM; and Ti + PLGA + SIM0.6%: Ti + PLGA incorporating 0.6% SIM. PLGA was prepared through chloroform evaporation technique. After complete dissolution of PLGA, SIM was diluted in the solution. Ti + PLGA, Ti + PLGA + SIM6%, and Ti + PLGA + SIM0.6% were dip coated with PLGA and PLGA + SIM, respectively. Samples were sterilized by ethylene oxide. For SIM release assay, disks were submerged in PBS, pH 7.4, 37°C, 30 rpm up to 600 hours. At different time intervals, SIM was quantified by spectrophotometry (238 nm). For characterization of the biomaterial components, it was performed Fourier‐transform infrared spectroscopy, differential scanning calorimetry, scanning electron microscopy (SEM), optical profilometry, and atomic force microscopy. Biocompatibility analyses were performed by MTS colorimetric assay on murine fibroblasts L929, human gingival fibroblasts (HGFs), and stem cells from human exfoliated deciduous teeth (SHEDs). Absorbance was measured at 490 nm, and percentages of viable cells were calculated in relation to positive control (Ti). SEM images were obtained to verify cell adhesion and morphology. One‐way ANOVA followed by Tukey's post hoc test was applied ( P < 0.05) for statistical analyses. Results SIM release was slow and continuous, reaching about 21% of the incorporated SIM after 600 hours. Topographical analyses revealed success in coating Ti disks with PLGA incorporating SIM. Regarding biocompatibility test, Ti + PLGA + SIM0.6% showed the highest percentage of L929 viability at days 3 and 7. There was no significant difference for Ti, Ti + PLGA, and Ti + PLGA + SIM0.6% groups on ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jre.12695 |
| الاتاحة: | http://dx.doi.org/10.1111/jre.12695 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjre.12695 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jre.12695 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jre.12695 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.C134B51A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jre.12695 |
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