Academic Journal
Molecular analysis of antigen recognition by insulin-specific T-cell hybridomas from B6 wild-type and bm12 mutant mice.
| العنوان: | Molecular analysis of antigen recognition by insulin-specific T-cell hybridomas from B6 wild-type and bm12 mutant mice. |
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| المؤلفون: | Sherman, D H, Hochman, P S, Dick, R, Tizard, R, Ramachandran, K L, Flavell, R A, Huber, B T |
| المصدر: | Molecular and Cellular Biology ; volume 7, issue 5, page 1865-1872 ; ISSN 0270-7306 1098-5549 |
| بيانات النشر: | Informa UK Limited |
| سنة النشر: | 1987 |
| الوصف: | Molecular analysis of the heterodimeric T-cell antigen receptor of insulin-specific class II-restricted T-cell hybridomas (THys) derived from C57BL/6 (B6) wild-type and B6.C-H-2bm12 (bm12) mutant mice revealed that such T cells use a diverse V gene repertoire. Analysis of three THys that use related V genes, however, showed a number of novel features. Two THys that share major histocompatibility complex restriction use V alpha genes that are 98.6% homologous. Two THys sharing the same antigen fine specificity use a particular germ line V beta D beta J beta combination. A 21-base-pair deletion in the 5' segment of the J beta gene occurs in one THy, suggesting a novel mechanism for generating diversity in T-cell antigen receptor beta genes. The first amino acid encoded by N sequences at the V-D junction is conserved in a pair of T cells which recognize identical antigenic epitopes. The implications of these findings for the structural mechanisms underlying major histocompatibility complex-restricted antigen-specific T-cell recognition are discussed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/mcb.7.5.1865 |
| DOI: | 10.1128/MCB.7.5.1865 |
| الاتاحة: | http://dx.doi.org/10.1128/mcb.7.5.1865 https://syndication.highwire.org/content/doi/10.1128/MCB.7.5.1865 |
| رقم الانضمام: | edsbas.C0F351E5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/mcb.7.5.1865 |
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