Academic Journal
Non-viral Smad7 gene delivery and attenuation of postoperative peritoneal adhesion in an experimental model
| العنوان: | Non-viral Smad7 gene delivery and attenuation of postoperative peritoneal adhesion in an experimental model |
|---|---|
| المؤلفون: | Guo, H, Leung, J C K, Cheung, J S, Chan, L Y Y, Wu, E X, Lai, K N |
| المساهمون: | Government Matching Grant Scheme, L & T Charitable Foundation, House of INDOCAFE |
| المصدر: | British Journal of Surgery ; volume 96, issue 11, page 1323-1335 ; ISSN 0007-1323 1365-2168 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2009 |
| الوصف: | Background Postoperative intra-abdominal adhesion is associated with high morbidity and mortality. Smad7, a protein that occupies a strategic position in fibrogenesis, inhibits the transforming growth factor (TGF) β/Smad signalling pathway. In this study the therapeutic potential of exogenous Smad7 in preventing fibrogenesis in postoperative intra-abdominal adhesion was investigated. Methods Intra-abdominal adhesion was induced in a rodent model by peritoneal abrasion. Smad7 was delivered into the peritoneal cavity by a non-viral ultrasound–microbubble-mediated naked gene transfection system. The effect of Smad7 transgene on adhesion formation was studied by measuring changes in TGF-β, fibrogenic factors, α-SMA and Smad2/3 activation in the anterior abdominal wall. Results Four weeks after surgical abrasion, all rats developed significant peritoneal adhesion with enhanced TGF-β expression, increased levels of extracellular matrix components and activated myofibroblasts, accompanied by decreased Smad7 expression and increased Smad2/3 activation. In rats treated with the Smad7 transgene, the incidence and severity of peritoneal adhesion were significantly reduced, with biochemical downregulation of fibrogenic factors and inhibition of Smad2/3 activation. Serial quantitation using magnetic resonance imaging revealed a significant reduction in adhesion areas from day 14 onwards. Conclusion Ultrasound–microbubble-mediated gene transfection provides timely targeted gene delivery for the treatment of postoperative peritoneal adhesions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/bjs.6722 |
| الاتاحة: | http://dx.doi.org/10.1002/bjs.6722 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbjs.6722 http://academic.oup.com/bjs/article-pdf/96/11/1323/36648280/bjs6722.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.C0C9877A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/bjs.6722 |
|---|