Academic Journal

P-234 The internal temperature of the catheter during the transfer: potential effects on the embryonic microenvironment

التفاصيل البيبلوغرافية
العنوان: P-234 The internal temperature of the catheter during the transfer: potential effects on the embryonic microenvironment
المؤلفون: Scarica, C, Moreno, S, Barbarulo, F, Bianco, C, Carconi, G, Sanchez, M, Gardini, F, Monaco, A
المصدر: Human Reproduction ; volume 39, issue Supplement_1 ; ISSN 0268-1161 1460-2350
بيانات النشر: Oxford University Press (OUP)
سنة النشر: 2024
الوصف: Study question Do the external environment and the different loading procedures affect temperature in the transfer catheter and embryo development during embryo transfer? Summary answer The inner temperature of transfer catheter dropped during the embryo transfer. Mouse 2-cell-embryos exposed to the transfer showed lower blastocyst and hatching rates. What is known already Embryo transfer is a crucial process composed of complex phases, which start from embryo loading into the catheter, and culminate in the transfer room in an open environment at room temperature. Although the transfer technique has been refined over time and several adjustments have been proposed towards its standardization (ASRM, 2017, D’Angelo et al., 2022), there is still great variability (Cirillo et al 2020). Few literature data are available regarding possible influences of the external environment on inner catheter conditions. Only recently, temperature drop during transfer has been described (Macklon et al 2021). Study design, size, duration We addressed possible temperature variations to which the embryo is exposed during transfer, by using a thin temperature probe placed inside a catheter. We simulated a total of 120 transfers (60 transfers with “fluid only” methodology and 60 with “air-fluid”). Additionally, we performed Mouse Embryo Assay (MEA) during transfer simulations and then cultured up to blastocyst to assess any impact of temperature drop exposure during transfer Participants/materials, setting, methods 120 mock transfers were performed, recording the temperature in the inner catheter were the embryos are loaded. In our setting, the procedure lasts 60-100 seconds. The internal temperature of the catheter was recorded after 15 min of preheating, at the loading and in the following 60 seconds. Environmental temperature and humidity values were recorded. A total of 73 mouse embryo were used for MEA (39 exposed to catheter, 34 controls) and cultured in time-lapse Main results and the role of chance The 120 simulations ...
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1093/humrep/deae108.604
الاتاحة: http://dx.doi.org/10.1093/humrep/deae108.604
https://academic.oup.com/humrep/article-pdf/39/Supplement_1/deae108.604/58399366/deae108.557.pdf
Rights: https://academic.oup.com/pages/standard-publication-reuse-rights
رقم الانضمام: edsbas.C0C227F5
قاعدة البيانات: BASE
الوصف
DOI:10.1093/humrep/deae108.604