Academic Journal
Solvent‐free liquid avidin as a step toward cold chain elimination
| العنوان: | Solvent‐free liquid avidin as a step toward cold chain elimination |
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| المؤلفون: | Bui‐Le, Liem, Brogan, Alex P. S., Hallett, Jason P. |
| المساهمون: | Engineering and Physical Sciences Research Council |
| المصدر: | Biotechnology and Bioengineering ; volume 118, issue 2, page 592-600 ; ISSN 0006-3592 1097-0290 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2020 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The temperature sensitivity of vaccines and therapeutic proteins forces the distribution of life‐saving treatments to rely heavily on the temperature‐controlled (usually 2–8°C) supply and distribution network known as the cold chain. Here, using avidin as a model, we demonstrate how surface engineering could significantly increase the thermal stability of therapeutic proteins. A combination of spectroscopic (Fourier transform infrared, circular dichroism, and ultraviolet‐visible) and scattering techniques (dynamic light scattering, small‐angle, and wide‐angle X‐ray scattering) were deployed to probe the activity, structure, and stability of the model protein. Temperature‐dependent synchrotron radiation circular dichroism spectroscopy was used to demonstrate a significant increase in thermal stability, with a half denaturation temperature of 139.0°C and reversible unfolding with modified avidin returning to a 90% folded state when heated to temperatures below 100°C. Accelerated aging studies revealed that modified avidin retained its secondary structure after storage at 40°C for 56 days, equivalent to 160 days at 25°C. Furthermore, binding studies with multiple ligands revealed that the binding site remained functional after modification. As a result, this approach has potential as a storage technology for therapeutic proteins and the elimination of the cold chain, enabling the dissemination of life‐saving vaccines worldwide. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/bit.27587 |
| الاتاحة: | http://dx.doi.org/10.1002/bit.27587 https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.27587 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.27587 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.C0683A38 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/bit.27587 |
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