Dissertation/ Thesis
Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma ; Strukturna i funkcionalna analiza hibridnog proteasoma
| العنوان: | Structural and functional analysis of hybrid proteasome Strukturna i funkcionalna analiza hibridnog proteasoma ; Strukturna i funkcionalna analiza hibridnog proteasoma |
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| المؤلفون: | Belačić, Katarina |
| المساهمون: | Haselbach, David, Malenica, Nenad |
| بيانات النشر: | Sveučilište u Zagrebu. Prirodoslovno-matematički fakultet. Biološki odsjek. University of Zagreb. Faculty of Science. Department of Biology. |
| سنة النشر: | 2018 |
| المجموعة: | Croatian Digital Theses Repository (National and University Library in Zagreb) |
| مصطلحات موضوعية: | 20S core particle, 19S regulatory particle, hybrid proteasome, cleavage specificities, cryo-electron microscop, 20S kompleks, 19S regulatorni kompleks, hibridni proteasom, specifičnosti proteolitičkog cijepanja, krio-elektronska mikroskopija, PRIRODNE ZNANOSTI. Biologija, NATURAL SCIENCES. Biology |
| الوصف: | Proteasomes perform the majority of proteolysis that occurs in cells, and thereby, perform crucial roles in regulation of cellular processes such as cell division and death, signal transduction, immune response, metabolism and protein quality control. The catalytic heart of proteasome complexes is the 20S core particle (20S CP) which activity is regulated through association with different activators – 19S regulatory particle (19SRP), PA28ab, PA28g and PA200. The hybrid proteasome complex contains the 19S RP on the one side and PA28 or PA200 on the opposite side of the same 20S CP. The hybrid proteasome complexes can be found under native conditions, or their formation can be induced (e.g., by interferon-γ treatment). The exact function of hybrid proteasomes is still unknown and their structures have never been thoroughly investigated. The structural analysis of hybrid proteasome complexes from bovine testes and interferon-g treated human rectal carcinoma cells by single particle cryo-electron microscopy has resulted only in the structures of the PA200 hybrid proteasome complex, however at a too low resolution for reliable structural insights. Meanwhile, the peptide pattern in the presence of 19S-20S-PA28ab or 19S-20S-PA28g complexes was found to be changed in comparison with the one obtained without PA28 complexes, indicating that PA28 allosterically regulates the cleavage specificities of 19S-20S proteasome complex. ; Proteasom je proteinski kompleks zadužen za razgradnju proteina unutar stanice te je stoga važan za regulaciju staničnih procesa – diobu i smrt stanice, prijenos signala, imunološki odgovor, metabolizam, provjeru kvalitete proteina i razvoj. Katalitički aktivan dio čini 20S kompleks čija je aktivnost regulirana vezanjem različitih aktivatora poput 19S regulatornog kompleksa (19S RP), PA28ab, PA28g te PA200. Hibridni proteasom sadrži 19S RP na jednoj i PA28 ili PA200 na drugoj strani istog 20S kompleksa. Hibridni proteasomi mogu biti prisutni u normalnim uvjetima ili njihov nastanak može biti ... |
| نوع الوثيقة: | master thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://zir.nsk.hr/islandora/object/pmf:5168; https://urn.nsk.hr/urn:nbn:hr:217:182054; https://repozitorij.unizg.hr/islandora/object/pmf:5168; https://repozitorij.unizg.hr/islandora/object/pmf:5168/datastream/PDF |
| الاتاحة: | https://zir.nsk.hr/islandora/object/pmf:5168 https://urn.nsk.hr/urn:nbn:hr:217:182054 https://repozitorij.unizg.hr/islandora/object/pmf:5168 https://repozitorij.unizg.hr/islandora/object/pmf:5168/datastream/PDF |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ ; info:eu-repo/semantics/embargoedAccess |
| رقم الانضمام: | edsbas.C049C959 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |