Academic Journal
A procedure to extract functional isolated mitochondria from small-sized human atrial samples. Application to obesity with a partial characterisation of the organelles
| العنوان: | A procedure to extract functional isolated mitochondria from small-sized human atrial samples. Application to obesity with a partial characterisation of the organelles |
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| المؤلفون: | Léger, Thibault, Jouve, Chrystele, Patrac, Véronique, Batel, Valerie, Bouvier, Damien, Sapin, Vincent, Miguel, Bruno, Demaison, Luc, Azarnoush, Kasra |
| المساهمون: | Unité de Nutrition Humaine (UNH), Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CHU Gabriel Montpied Clermont-Ferrand, CHU Clermont-Ferrand, French Federation of Cardiology (FFC) surgical association for the development and enhancement of the techniques of screening and treatment of cardiovascular diseases |
| المصدر: | ISSN: 0891-5849 ; Free Radical Biology and Medicine ; https://hal.inrae.fr/hal-02623749 ; Free Radical Biology and Medicine, 2020, 153, pp.71-79. ⟨10.1016/j.freeradbiomed.2020.04.006⟩. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Institut National de la Recherche Agronomique: ProdINRA |
| مصطلحات موضوعية: | obésité, maladie coronarienne, mitochondrie, diagnostic précoce, Méthodologie d'extraction, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience ; Evaluating the activity of cardiac mitochondria is probably the best way to estimate early cellular damage in chronic pathology. Early diagnosis allows rapid therapeutic intervention thus increasing patient survival rate in a number of diseases. However, data on human cardiac mitochondria are scarce in the international literature. Here, we describe a method to extract and study functional mitochondria from the small-sized right atrial aliquots (minimum of 400 mg) obtained during extracorporeal circulation and usually considered as surgical waste products. The mitochondria were purified through several mechanical processes (fine myocardial cutting, tissue grinding and potter Elvehjem homogenising), an enzymatic proteolytic action (subtilisin) and differential centrifugations. In chronic pathologies, including obesity, early disturbances of mitochondrial function can occur. The effects of obesity on the rate of mitochondrial oxygen consumption and H2O2 release were thus determined with three different substrates (glutamate/malate, succinate/rotenone and palmitoylcarnitine/malate). The human atrial mitochondria were of high quality from a functional viewpoint, compared to rat ventricle organelles, but the extraction yield of the human mitochondria was twice lower than that of rat mitochondria. Tests showed that glutamate/malate-related ADP-stimulated respiration was strongly increased in obese subjects, although the oxidation of the other two substrates was unaffected. Reactive oxygen species (ROS) production by the isolated mitochondria was low in comparison with that of the lean subjects. These results confirm those found in one of our previous studies in the ventricles of rats fed a high-fat diet. In conclusion, the described method is simple, reliable and sensitive. It allows for the description of the impact of obesity on the function of atrial mitochondria while using only a small patient sampling (n = 5 in both the lean and the obese groups). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-537131-2 0-00-537131-7 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32330586; PUBMED: 32330586; WOS: 000537131700007 |
| DOI: | 10.1016/j.freeradbiomed.2020.04.006 |
| الاتاحة: | https://hal.inrae.fr/hal-02623749 https://doi.org/10.1016/j.freeradbiomed.2020.04.006 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ |
| رقم الانضمام: | edsbas.C00D55E9 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780005371312 0005371317 |
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| DOI: | 10.1016/j.freeradbiomed.2020.04.006 |