Academic Journal
Mesenchyme instructs growth while epithelium directs branching in the mouse mammary gland
| العنوان: | Mesenchyme instructs growth while epithelium directs branching in the mouse mammary gland |
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| المؤلفون: | Qiang Lan, Ewelina Trela, Riitta Lindström, Jyoti Prabha Satta, Beata Kaczyńska, Mona M Christensen, Martin Holzenberger, Jukka Jernvall, Marja L Mikkola |
| المصدر: | eLife, Vol 13 (2024) |
| بيانات النشر: | eLife Sciences Publications Ltd |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | mammary gland, epithelial-mesenchymal interaction, branching morphogenesis, ectodysplasin A, Wnt, IGF-1, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The mammary gland is a unique organ that undergoes dynamic alterations throughout a female’s reproductive life, making it an ideal model for developmental, stem cell and cancer biology research. Mammary gland development begins in utero and proceeds via a quiescent bud stage before the initial outgrowth and subsequent branching morphogenesis. How mammary epithelial cells transit from quiescence to an actively proliferating and branching tissue during embryogenesis and, importantly, how the branch pattern is determined remain largely unknown. Here, we provide evidence indicating that epithelial cell proliferation and onset of branching are independent processes, yet partially coordinated by the Eda signaling pathway. Through heterotypic and heterochronic epithelial-mesenchymal recombination experiments between mouse mammary and salivary gland tissues and ex vivo live imaging, we demonstrate that unlike previously concluded, the mode of branching is an intrinsic property of the mammary epithelium whereas the pace of growth and the density of ductal tree are determined by the mesenchyme. Transcriptomic profiling and ex vivo and in vivo functional studies in mice disclose that mesenchymal Wnt/ß-catenin signaling, and in particular IGF-1 downstream of it critically regulate mammary gland growth. These results underscore the general need to carefully deconstruct the different developmental processes producing branched organs. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/93326; https://doaj.org/toc/2050-084X; e93326; https://doaj.org/article/7481288197854bb1bc630a34b23a8629 |
| DOI: | 10.7554/eLife.93326 |
| الاتاحة: | https://doi.org/10.7554/eLife.93326 https://doaj.org/article/7481288197854bb1bc630a34b23a8629 |
| رقم الانضمام: | edsbas.BFE9D04B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.93326 |