Academic Journal
Differential response of olfactory sensory neuron populations to copper ion exposure in zebrafish
| العنوان: | Differential response of olfactory sensory neuron populations to copper ion exposure in zebrafish |
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| المؤلفون: | LAZZARI, MAURIZIO, BETTINI, SIMONE, MILANI, LILIANA, MAURIZII, MARIA GABRIELLA, FRANCESCHINI, VALERIA |
| المساهمون: | Lazzari, Maurizio, Bettini, Simone, Milani, Liliana, Maurizii, Maria Gabriella, Franceschini, Valeria |
| سنة النشر: | 2017 |
| المجموعة: | IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| مصطلحات موضوعية: | Copper ion, Olfactory epithelium, Olfactory sensory neuron, Crypt cell, Olfactory recovery, Zebrafish, Immunohistochemistry |
| الوصف: | The peripheral olfactory system of fish is in direct contact with the external aqueous environment, so dissolved contaminants can easily impair sensory functions and cause neurobehavioral injuries. The olfactory epithelium of fish is arranged in lamellae forming a rosette in the olfactory cavity and contains three main types of olfactory sensory neurons (OSNs): ciliated (cOSNs) and microvillous olfactory sensory neurons (mOSNs), common to all vertebrates, and a third minor group of olfactory neurons, crypt cells, absent in tetrapods. Since copper is a ubiquitously diffusing olfactory toxicant and a spreading contaminant in urban runoff, we investigated the effect of low copper concentration on the three different OSNs in the olfactory epithelium of zebrafish, a model system widely used in biological research. Image analysis was applied for morphometry and quantification of immunohistochemically detected OSNs. Copper exposure resulted in an evident decrease in olfactory epithelium thickness. Moreover, after exposure, the lamellae of the dorsal and ventral halves of the olfactory rosettes showed a different increase in their sensory areas, suggesting a lateral migration of new cells into non-sensory regions. The results of the present study provide clear evidence of a differential response of the three neural cell populations of zebrafish olfactory mucosa after 96 h of exposure to copper ions at the sublethal concentration of 30 μg/L-1. Densitometric values of cONS, immunostained with anti-G αolf, decreased of about 60% compared to the control. When the fish were transferred to water without copper addition and examined after 3, 10 and 30 days, we observed a partial restoration of anti-Gαolf staining intensity to normal condition. The recovery of cOSNs appeared sustained by neuronal proliferation, quantified with anti-PCNA immunostaining, in particular in the early days after exposure. The densitometric analysis applied to mOSNs, immunostained with anti-TRPC2, revealed a statistically significant decrease of about ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/27992776; info:eu-repo/semantics/altIdentifier/wos/WOS:000394071100007; volume:183; firstpage:54; lastpage:62; numberofpages:9; journal:AQUATIC TOXICOLOGY; http://hdl.handle.net/11585/607597; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85006516039; http://www.elsevier.com/wps/find/journaldescription.cws_home/505509/description#description |
| DOI: | 10.1016/j.aquatox.2016.12.012 |
| الاتاحة: | http://hdl.handle.net/11585/607597 https://doi.org/10.1016/j.aquatox.2016.12.012 http://www.elsevier.com/wps/find/journaldescription.cws_home/505509/description#description |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BFE49B09 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.aquatox.2016.12.012 |
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