Academic Journal
Enhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications
| العنوان: | Enhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications |
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| المؤلفون: | Ateş, Burhan, Ulu, Ahmet, Asiltürk, Meltem, Noma, Samir Abbas Ali, Demirel Topel, Seda, Dik, Gamze, Özhan, Onural, Bakar, Büşra, Yıldız, Azibe, Vardı, Nigar, Parlakpınar, Hakan |
| المساهمون: | 0000-0002-0567-5627 Demirel Topel, Seda, Demirel Topel, Seda, 280190 Demirel Topel, Seda, 55225915900 Demirel Topel, Seda |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Enzyme immobilization, PEG-L-asparaginase, Upconverting nanoparticles (UCNPs), Near-infrared (NIR) excitation, Triggered activity, Enzim immobilizasyonu, PEG-L-asparaginaz, Nanopartiküllerin yukarı dönüştürülmesi (UCNP'ler), Yakın kızılötesi (NIR) uyarım, Tetiklenen etkinlik |
| الوصف: | If the appropriate immobilization method and carrier support are not selected, partial decreases in the activity of enzymes may occur after immobilization. Herein, to overcome this challenge, an excitation mechanism that enables energy transfer was proposed. Modified upconverting nanoparticles (UCNPs) were constructed and the important role of near-infrared (NIR) excitation in enhancing the catalytic activity of the enzyme was demonstrated. For this purpose, UCNPs were first synthesized via the hydrothermal method, functionalized with isocyanate groups, and then, PEG-L-ASNase was immobilized via covalent binding. UCNPs with and without PEG-L- ASNase were extensively characterized by different methods. These supports had immobilization yield and activity efficiency of >96 % and 78 %, respectively. Moreover, immobilized enzymes exhibited improved pH, thermal, and storage stability. In addition, they retained >65 % of their initial activity even after 20 catalytic cycles. Biochemical and histological findings did not indicate a trend of toxicity in rats due to UCNPs. Most importantly, PEG-L-ASNase activity was triggered approximately 5- and 2-fold under in vitro and in vivo conditions, respectively. Overall, it is anticipated that this pioneering work will shed new light on the realistic and promising usage of NIR-excited UCNPs for the immobilization of enzymes in expensive and extensive applications. ; No sponsor |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0141-8130 |
| Relation: | International publication; International Journal of Biological Macromolecules; Ateş, B., Ulu, A., Asiltürk, M., Noma, S. A. A., Demirel Topel, S., Dik, G., Özhan, O., Bakar, B., Yıldız, A., Vardı, N. & Parlakpınar, H. (2024). Enhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications. International Journal of Biological Macromolecules, 260.; http://hdl.handle.net/20.500.12566/2081; WOS:001174291500001; 2-s2.0-85183315524; 260 |
| DOI: | 10.1016/j.ijbiomac.2024.129343 |
| الاتاحة: | https://hdl.handle.net/20.500.12566/2081 https://doi.org/10.1016/j.ijbiomac.2024.129343 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.BFA29959 |
| قاعدة البيانات: | BASE |
| تدمد: | 01418130 |
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| DOI: | 10.1016/j.ijbiomac.2024.129343 |