Academic Journal
SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation
| العنوان: | SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation |
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| المؤلفون: | Winterhalter, C, Stevens, D, Fenyk, S, Pelliciari, S, Marchand, E, Soultanas, P, Ilangovan, A, Murray, H |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2023 |
| المجموعة: | Queen Mary University of London: Queen Mary Research Online (QMRO) |
| الوصف: | Bidirectional DNA replication from a chromosome origin requires the asymmetric loading of two helicases, one for each replisome. Our understanding of the molecular mechanisms underpinning helicase loading at bacterial chromosome origins is incomplete. Here we report both positive and negative mechanisms for directing helicase recruitment in the model organism Bacillus subtilis. Systematic characterization of the essential initiation protein DnaD revealed distinct protein interfaces required for homo-oligomerization, interaction with the master initiator protein DnaA, and interaction with the helicase co-loader protein DnaB. Informed by these properties of DnaD, we went on to find that the developmentally expressed repressor of DNA replication initiation, SirA, blocks the interaction between DnaD and DnaA, thereby restricting helicase recruitment from the origin during sporulation to inhibit further initiation events. These results advance our understanding of the mechanisms underpinning DNA replication initiation in B. subtilis, as well as guiding the search for essential cellular activities to target for antimicrobial drug design. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 4302 - 4321 |
| اللغة: | unknown |
| تدمد: | 0305-1048 |
| Relation: | NUCLEIC ACIDS RESEARCH; https://qmro.qmul.ac.uk/xmlui/handle/123456789/91808 |
| DOI: | 10.1093/nar/gkac1060 |
| الاتاحة: | https://qmro.qmul.ac.uk/xmlui/handle/123456789/91808 https://doi.org/10.1093/nar/gkac1060 |
| Rights: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. ; Attribution 3.0 United States ; http://creativecommons.org/licenses/by/3.0/us/ ; © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. |
| رقم الانضمام: | edsbas.BF5531 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 03051048 |
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| DOI: | 10.1093/nar/gkac1060 |