Academic Journal
Identification of Mutant Genes and Introgressed Tiger Salamander DNA in the Laboratory Axolotl, Ambystoma mexicanum
| العنوان: | Identification of Mutant Genes and Introgressed Tiger Salamander DNA in the Laboratory Axolotl, Ambystoma mexicanum |
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| المؤلفون: | Woodcock, M. Ryan, Vaughn-Wolfe, Jennifer, Elias, Alexandra, Kump, D. Kevin, Kendall, Katharina Denise, Timoshevskaya, Nataliya, Timoshevskiy, Vladimir, Perry, Dustin W., Smith, Jeramiah J., Spiewak, Jessica E., Parichy, David M., Voss, S. Randal |
| المصدر: | Scientific Reports ; volume 7, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2017 |
| الوصف: | The molecular genetic toolkit of the Mexican axolotl, a classic model organism, has matured to the point where it is now possible to identify genes for mutant phenotypes. We used a positional cloning–candidate gene approach to identify molecular bases for two historic axolotl pigment phenotypes: white and albino. White ( d / d ) mutants have defects in pigment cell morphogenesis and differentiation, whereas albino ( a / a ) mutants lack melanin. We identified in white mutants a transcriptional defect in endothelin 3 ( edn3 ), encoding a peptide factor that promotes pigment cell migration and differentiation in other vertebrates. Transgenic restoration of Edn3 expression rescued the homozygous white mutant phenotype. We mapped the albino locus to tyrosinase ( tyr ) and identified polymorphisms shared between the albino allele ( tyr a ) and tyr alleles in a Minnesota population of tiger salamanders from which the albino trait was introgressed. tyr a has a 142 bp deletion and similar engineered alleles recapitulated the albino phenotype. Finally, we show that historical introgression of tyr a significantly altered genomic composition of the laboratory axolotl, yielding a distinct, hybrid strain of ambystomatid salamander. Our results demonstrate the feasibility of identifying genes for traits in the laboratory Mexican axolotl. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-017-00059-1 |
| الاتاحة: | http://dx.doi.org/10.1038/s41598-017-00059-1 https://www.nature.com/articles/s41598-017-00059-1.pdf https://www.nature.com/articles/s41598-017-00059-1 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.BF40C9DE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-017-00059-1 |
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