التفاصيل البيبلوغرافية
| العنوان: |
Native Electrospray Ionization of Multi-Domain Proteins via a Bead Ejection Mechanism |
| المؤلفون: |
Nina Khristenko (14304089), Frédéric Rosu (1342200), Eric Largy (1901707), Jérôme Haustant (14304092), Cédric Mesmin (1643554), Valérie Gabelica (1342197) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Molecular Biology, Biotechnology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, wherein globular domains, suggesting several conformations, domain proteins via, charged residue mechanism, native electrospray ionization, intrinsically disordered proteins, ionization mechanism, disordered linkers, biomolecule electrospray, hybrid mechanism, potentially useful, phase conformation, parent droplet, intriguing question, general tenet, ejected one, deuterium exchange, cannot ionize, biophysical characterization, also compatible |
| الوصف: |
Native ion mobility mass spectrometry is potentially useful for the biophysical characterization of proteins, as the electrospray charge state distribution and the collision cross section distribution depend on their solution conformation. We examine here the charging and gas-phase conformation of multi-domain therapeutic proteins comprising globular domains tethered by disordered linkers. The charge and collision cross section distributions are multimodal, suggesting several conformations in solution, as confirmed by solution hydrogen/deuterium exchange. The most intriguing question is the ionization mechanism of these structures: a fraction of the population does not follow the charged residue mechanism but cannot ionize by pure chain ejection either. We deduce that a hybrid mechanism is possible, wherein globular domains are ejected one at a time from a parent droplet. The charge vs solvent accessible surface area correlations of denatured and intrinsically disordered proteins are also compatible with this “bead ejection mechanism”, which we propose as a general tenet of biomolecule electrospray. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Native_Electrospray_Ionization_of_Multi-Domain_Proteins_via_a_Bead_Ejection_Mechanism/21782471 |
| DOI: |
10.1021/jacs.2c10762.s001 |
| الاتاحة: |
https://doi.org/10.1021/jacs.2c10762.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.BF4094CE |
| قاعدة البيانات: |
BASE |