Academic Journal
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry.
| العنوان: | Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry. |
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| المؤلفون: | Depierreux, Delphine M, Seshadri, Emily, Shmeleva, Evgeniya V, Kieckbusch, Jens, Hawkes, Delia A, Colucci, Francesco |
| بيانات النشر: | MyJove Corporation //doi.org/10.3791/62670 J Vis Exp |
| سنة النشر: | 2021 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Animals, Female, Flow Cytometry, Immunity, Innate, Lymphocytes, Mice, Phenotype, Pregnancy, Uterus |
| الوصف: | Described here is a simple method to isolate and phenotype mouse group 1 uterine innate lymphoid cells (g1 uILCs) from individual pregnant uterus by flow cytometry. The protocol describes how to set up time mating to obtain multiple synchronous dams, the mechanical and enzymatic digestion of the pregnant uterus, the staining of single-cell suspensions, and a FACS strategy to phenotype and discriminate g1 uILCs. Although this method inevitably loses the spatial information of cellular distribution within the tissue, the protocol has been successfully applied to determine uILC heterogeneity, their response to maternal and foetal factors affecting pregnancy, their gene expression profile, and their functions. ; Our lab is supported by a Wellcome Investigator Award (200841/Z/16/Z), and an MRC project grant (MR/P001092/1) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| اللغة: | English |
| Relation: | https://www.repository.cam.ac.uk/handle/1810/322433 |
| DOI: | 10.17863/CAM.69891 |
| الاتاحة: | https://www.repository.cam.ac.uk/handle/1810/322433 https://doi.org/10.17863/CAM.69891 |
| Rights: | All rights reserved |
| رقم الانضمام: | edsbas.BEDD8827 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.69891 |
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