Academic Journal
The GDNF-GFRα1 complex promotes the development of hippocampal dendritic arbors and spines via NCAM.
| العنوان: | The GDNF-GFRα1 complex promotes the development of hippocampal dendritic arbors and spines via NCAM. |
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| المؤلفون: | Irala, Dolores, Bonafina, Antonela, Fontanet, Paula Aldana, Alsina, Fernando Cruz, Paratcha, Gustavo, Ledda, Fernanda |
| المصدر: | Development (Cambridge, England), 143 (22), 4224-4235 (2016) |
| سنة النشر: | 2016 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | GDNF, Hippocampus, neuronal plasticity, Life sciences, Anatomy (cytology, histology, embryology.) & physiology, Sciences du vivant, Anatomie (cytologie, histologie, embryologie.) & physiologie |
| الوصف: | peer reviewed ; The formation of synaptic connections during nervous system development requires the precise control of dendrite growth and synapse formation. Although glial cell line-derived neurotrophic factor (GDNF) and its receptor GFRα1 are expressed in the forebrain, the role of this system in the hippocampus remains unclear. Here, we investigated the consequences of GFRα1 deficiency for the development of hippocampal connections. Analysis of conditional Gfra1 knockout mice shows a reduction in dendritic length and complexity, as well as a decrease in postsynaptic density specializations and in the synaptic localization of postsynaptic proteins in hippocampal neurons. Gain- and loss-of-function assays demonstrate that the GDNF-GFRα1 complex promotes dendritic growth and postsynaptic differentiation in cultured hippocampal neurons. Finally, in vitro assays revealed that GDNF-GFRα1-induced dendrite growth and spine formation are mediated by NCAM signaling. Taken together, our results indicate that the GDNF-GFRα1 complex is essential for proper hippocampal circuit development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0950-1991 1477-9129 |
| Relation: | urn:issn:0950-1991; urn:issn:1477-9129; https://orbi.uliege.be/handle/2268/248244; info:hdl:2268/248244; https://orbi.uliege.be/bitstream/2268/248244/1/Bonafina%20development.pdf; info:pmid:27707798 |
| DOI: | 10.1242/dev.140350 |
| الاتاحة: | https://orbi.uliege.be/handle/2268/248244 https://orbi.uliege.be/bitstream/2268/248244/1/Bonafina%20development.pdf https://doi.org/10.1242/dev.140350 |
| Rights: | open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BDF42CD0 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 09501991 14779129 |
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| DOI: | 10.1242/dev.140350 |