Academic Journal
A novel function for the atypical small G protein Rab-like 5 in the assembly of the trypanosome flagellum
| العنوان: | A novel function for the atypical small G protein Rab-like 5 in the assembly of the trypanosome flagellum |
|---|---|
| المؤلفون: | Adhiambo, Christine, Blisnick, Thierry, Toutirais, Géraldine, Delannoy, Emmanuelle, Bastin, Philippe |
| المساهمون: | Biologie Cellulaire des Trypanosomes, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Régulation et dynamique des génomes, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0021-9533. |
| بيانات النشر: | HAL CCSD Company of Biologists |
| سنة النشر: | 2009 |
| المجموعة: | Muséum National d'Histoire Naturelle (MNHM): HAL |
| مصطلحات موضوعية: | MESH: Amino Acid Sequence, MESH: Animals, MESH: Trypanosoma brucei brucei, MESH: Cilia, MESH: Flagella, MESH: Gene Silencing, MESH: Molecular Sequence Data, MESH: Monomeric GTP-Binding Proteins, MESH: Mutation, MESH: Protein Transport, MESH: Protozoan Proteins, MESH: Sequence Alignment, MESH: Sequence Homology, Amino Acid, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] |
| الوصف: | International audience ; The atypical small G protein Rab-like 5 has been shown to traffic in sensory cilia of Caenorhabditis elegans, where it participates in signalling processes but not in cilia construction. In this report, we demonstrate that RABL5 colocalises with intraflagellar transport (IFT) proteins at the basal body and in the flagellum matrix of the protist Trypanosoma brucei. RABL5 fused to GFP exhibits anterograde movement in the flagellum of live trypanosomes, suggesting it could be associated with IFT. Accordingly, RABL5 accumulates in the short flagella of the retrograde IFT140RNAi mutant and is restricted to the basal body region in the IFT88RNAi anterograde mutant, a behaviour that is identical to other IFT proteins. Strikingly, RNAi silencing reveals an essential role for RABL5 in trypanosome flagellum construction. RNAi knock-down produces a phenotype similar to inactivation of retrograde IFT with formation of short flagella that are filled with a high amount of IFT proteins. These data reveal for the first time a functional difference for a conserved flagellar matrix protein between two different ciliated species and raise questions related to cilia diversity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19240117; hal-03246322; https://hal.science/hal-03246322; https://hal.science/hal-03246322/document; https://hal.science/hal-03246322/file/834.pdf; PUBMED: 19240117 |
| DOI: | 10.1242/jcs.040444 |
| الاتاحة: | https://hal.science/hal-03246322 https://hal.science/hal-03246322/document https://hal.science/hal-03246322/file/834.pdf https://doi.org/10.1242/jcs.040444 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.BDF2E875 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/jcs.040444 |
|---|